Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100007 |
Resumo: | PURPOSE: To investigate the influence of intravenous nonselective cyclooxygenase inhibitor, ketoprofen (keto), on kidney histological changes and kidney cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), levels after hemorrhage of 30% of volemia (three times 10%, intervals of 10 min) in rats. METHODS: Under sevoflurane (sevo) anesthesia, sevo and sevo+keto groups (10 rats each) were instrumented for Ringer solution (5mL/kg/h) administration and mean arterial pressure (MAP) evaluation, plus keto (1.5mg/kg) administration in sevo+keto group in the beginning of anesthesia. Rectal temperature was continuously measured. The baseline data of temperature and MAP were collected at the first hemorrhage (T1), the third hemorrhage (T2) and 30min after T2 (T3). Bilateral nephrectomy was achieved for histology and immunohistochemistry. RESULTS: In both groups, temperature and MAP diminished from initial values. Hypothermia was greater in sevo group (p=0.0002). Tubular necrosis was more frequent in sevo group (p=0.02). The studied cytokines were equally present in the kidneys of both groups. CONCLUSION: Ketoprofen was more protective to the rat kidney in condition of anesthesia with sevoflurane and hypovolemia, but it seems that TNF-α and IL-1 were not involved in that protection. |
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Acta Cirúrgica Brasileira (Online) |
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Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effectKetoprofenHemorrhageKidneyCytokinesNecrosisRatsPURPOSE: To investigate the influence of intravenous nonselective cyclooxygenase inhibitor, ketoprofen (keto), on kidney histological changes and kidney cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), levels after hemorrhage of 30% of volemia (three times 10%, intervals of 10 min) in rats. METHODS: Under sevoflurane (sevo) anesthesia, sevo and sevo+keto groups (10 rats each) were instrumented for Ringer solution (5mL/kg/h) administration and mean arterial pressure (MAP) evaluation, plus keto (1.5mg/kg) administration in sevo+keto group in the beginning of anesthesia. Rectal temperature was continuously measured. The baseline data of temperature and MAP were collected at the first hemorrhage (T1), the third hemorrhage (T2) and 30min after T2 (T3). Bilateral nephrectomy was achieved for histology and immunohistochemistry. RESULTS: In both groups, temperature and MAP diminished from initial values. Hypothermia was greater in sevo group (p=0.0002). Tubular necrosis was more frequent in sevo group (p=0.02). The studied cytokines were equally present in the kidneys of both groups. CONCLUSION: Ketoprofen was more protective to the rat kidney in condition of anesthesia with sevoflurane and hypovolemia, but it seems that TNF-α and IL-1 were not involved in that protection.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100007Acta Cirúrgica Brasileira v.27 n.1 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012000100007info:eu-repo/semantics/openAccessGuedes Jr,Francisco SobreiraCruz,Deyvid Santos daRodrigues,Marcela Marcondes PintoSilva,Leopoldo Muniz daAmorim,Renée LauferVianna,Pedro Thadeu GalvãoCastiglia,Yara Marcondes Machadoeng2011-12-19T00:00:00Zoai:scielo:S0102-86502012000100007Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2011-12-19T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
title |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
spellingShingle |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect Guedes Jr,Francisco Sobreira Ketoprofen Hemorrhage Kidney Cytokines Necrosis Rats |
title_short |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
title_full |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
title_fullStr |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
title_full_unstemmed |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
title_sort |
Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect |
author |
Guedes Jr,Francisco Sobreira |
author_facet |
Guedes Jr,Francisco Sobreira Cruz,Deyvid Santos da Rodrigues,Marcela Marcondes Pinto Silva,Leopoldo Muniz da Amorim,Renée Laufer Vianna,Pedro Thadeu Galvão Castiglia,Yara Marcondes Machado |
author_role |
author |
author2 |
Cruz,Deyvid Santos da Rodrigues,Marcela Marcondes Pinto Silva,Leopoldo Muniz da Amorim,Renée Laufer Vianna,Pedro Thadeu Galvão Castiglia,Yara Marcondes Machado |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Guedes Jr,Francisco Sobreira Cruz,Deyvid Santos da Rodrigues,Marcela Marcondes Pinto Silva,Leopoldo Muniz da Amorim,Renée Laufer Vianna,Pedro Thadeu Galvão Castiglia,Yara Marcondes Machado |
dc.subject.por.fl_str_mv |
Ketoprofen Hemorrhage Kidney Cytokines Necrosis Rats |
topic |
Ketoprofen Hemorrhage Kidney Cytokines Necrosis Rats |
description |
PURPOSE: To investigate the influence of intravenous nonselective cyclooxygenase inhibitor, ketoprofen (keto), on kidney histological changes and kidney cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), levels after hemorrhage of 30% of volemia (three times 10%, intervals of 10 min) in rats. METHODS: Under sevoflurane (sevo) anesthesia, sevo and sevo+keto groups (10 rats each) were instrumented for Ringer solution (5mL/kg/h) administration and mean arterial pressure (MAP) evaluation, plus keto (1.5mg/kg) administration in sevo+keto group in the beginning of anesthesia. Rectal temperature was continuously measured. The baseline data of temperature and MAP were collected at the first hemorrhage (T1), the third hemorrhage (T2) and 30min after T2 (T3). Bilateral nephrectomy was achieved for histology and immunohistochemistry. RESULTS: In both groups, temperature and MAP diminished from initial values. Hypothermia was greater in sevo group (p=0.0002). Tubular necrosis was more frequent in sevo group (p=0.02). The studied cytokines were equally present in the kidneys of both groups. CONCLUSION: Ketoprofen was more protective to the rat kidney in condition of anesthesia with sevoflurane and hypovolemia, but it seems that TNF-α and IL-1 were not involved in that protection. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502012000100007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.27 n.1 2012 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126440865792000 |