The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000500334 |
Resumo: | Abstract Purpose: To investigate the glomerular number after different warm ischemia times. Methods: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia. The sham group underwent the same surgery without renal ischemia. The animals were euthanized after 3 weeks, and the kidneys were collected. Right kidneys were used as controls. The kidney weight, volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted mean glomerular volume, and the total number of glomeruli per kidney were obtained. Serum creatinine levels were assessed pre and postoperatively. Results: Serum creatinine levels did not differ among the groups. All parameters were similar for the sham, G10, and G20 groups upon comparison of the right and left organs. The G30 group pigs' left kidneys had lower weight, volume, and cortical-medullar ratio and 24.6% less glomeruli compared to the right kidney. A negative correlation was found between warm ischemia time and glomerular number. Conclusions: About one quarter of glomeruli was lost after 30 minutes of renal warm ischemia. No glomeruli loss was detected before 20 minutes of warm ischemia. However, progressive glomerular loss was associated with increasing warm ischemia time. |
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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig modelKidneyNephronsNephrectomyWarm IschemiaSwineAbstract Purpose: To investigate the glomerular number after different warm ischemia times. Methods: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia. The sham group underwent the same surgery without renal ischemia. The animals were euthanized after 3 weeks, and the kidneys were collected. Right kidneys were used as controls. The kidney weight, volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted mean glomerular volume, and the total number of glomeruli per kidney were obtained. Serum creatinine levels were assessed pre and postoperatively. Results: Serum creatinine levels did not differ among the groups. All parameters were similar for the sham, G10, and G20 groups upon comparison of the right and left organs. The G30 group pigs' left kidneys had lower weight, volume, and cortical-medullar ratio and 24.6% less glomeruli compared to the right kidney. A negative correlation was found between warm ischemia time and glomerular number. Conclusions: About one quarter of glomeruli was lost after 30 minutes of renal warm ischemia. No glomeruli loss was detected before 20 minutes of warm ischemia. However, progressive glomerular loss was associated with increasing warm ischemia time.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2017-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000500334Acta Cirúrgica Brasileira v.32 n.5 2017reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020170050000002info:eu-repo/semantics/openAccessDamasceno-Ferreira,José AurelinoBechara,Gustavo RuschiCosta,Waldemar SilvaPereira-Sampaio,Marco AurélioSampaio,Francisco José BarcellosSouza,Diogo Benchimol Deeng2017-06-02T00:00:00Zoai:scielo:S0102-86502017000500334Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2017-06-02T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
title |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
spellingShingle |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model Damasceno-Ferreira,José Aurelino Kidney Nephrons Nephrectomy Warm Ischemia Swine |
title_short |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
title_full |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
title_fullStr |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
title_full_unstemmed |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
title_sort |
The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model |
author |
Damasceno-Ferreira,José Aurelino |
author_facet |
Damasceno-Ferreira,José Aurelino Bechara,Gustavo Ruschi Costa,Waldemar Silva Pereira-Sampaio,Marco Aurélio Sampaio,Francisco José Barcellos Souza,Diogo Benchimol De |
author_role |
author |
author2 |
Bechara,Gustavo Ruschi Costa,Waldemar Silva Pereira-Sampaio,Marco Aurélio Sampaio,Francisco José Barcellos Souza,Diogo Benchimol De |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Damasceno-Ferreira,José Aurelino Bechara,Gustavo Ruschi Costa,Waldemar Silva Pereira-Sampaio,Marco Aurélio Sampaio,Francisco José Barcellos Souza,Diogo Benchimol De |
dc.subject.por.fl_str_mv |
Kidney Nephrons Nephrectomy Warm Ischemia Swine |
topic |
Kidney Nephrons Nephrectomy Warm Ischemia Swine |
description |
Abstract Purpose: To investigate the glomerular number after different warm ischemia times. Methods: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia. The sham group underwent the same surgery without renal ischemia. The animals were euthanized after 3 weeks, and the kidneys were collected. Right kidneys were used as controls. The kidney weight, volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted mean glomerular volume, and the total number of glomeruli per kidney were obtained. Serum creatinine levels were assessed pre and postoperatively. Results: Serum creatinine levels did not differ among the groups. All parameters were similar for the sham, G10, and G20 groups upon comparison of the right and left organs. The G30 group pigs' left kidneys had lower weight, volume, and cortical-medullar ratio and 24.6% less glomeruli compared to the right kidney. A negative correlation was found between warm ischemia time and glomerular number. Conclusions: About one quarter of glomeruli was lost after 30 minutes of renal warm ischemia. No glomeruli loss was detected before 20 minutes of warm ischemia. However, progressive glomerular loss was associated with increasing warm ischemia time. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000500334 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000500334 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0102-865020170050000002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.32 n.5 2017 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
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1752126444164612096 |