Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de entomologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400010 |
Resumo: | Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus. The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples may indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fst) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among populations points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them to respond differently to control measures. |
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Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphismBanded pine weevilInsectamolecular markerpopulation geneticsGenetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus. The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples may indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fst) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among populations points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them to respond differently to control measures.Sociedade Brasileira De Entomologia2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400010Revista Brasileira de Entomologia v.57 n.4 2013reponame:Revista brasileira de entomologia (Online)instname:Sociedade Brasileira De Entomologia (SBE)instacron:SBE10.1590/S0085-56262013005000036info:eu-repo/semantics/openAccessZaleski,Scheila R. M.Lazzari,Sonia M. N.Lazzarotto,Crisleide M.Panzavolta,TizianaIede,Edson T.Marques,Francisco de A.eng2014-01-10T00:00:00Zoai:scielo:S0085-56262013000400010Revistahttp://www.rbentomologia.com/https://old.scielo.br/oai/scielo-oai.php||sbe@ufpr.br1806-96650085-5626opendoar:2014-01-10T00:00Revista brasileira de entomologia (Online) - Sociedade Brasileira De Entomologia (SBE)false |
dc.title.none.fl_str_mv |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
title |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
spellingShingle |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism Zaleski,Scheila R. M. Banded pine weevil Insecta molecular marker population genetics |
title_short |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
title_full |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
title_fullStr |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
title_full_unstemmed |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
title_sort |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism |
author |
Zaleski,Scheila R. M. |
author_facet |
Zaleski,Scheila R. M. Lazzari,Sonia M. N. Lazzarotto,Crisleide M. Panzavolta,Tiziana Iede,Edson T. Marques,Francisco de A. |
author_role |
author |
author2 |
Lazzari,Sonia M. N. Lazzarotto,Crisleide M. Panzavolta,Tiziana Iede,Edson T. Marques,Francisco de A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Zaleski,Scheila R. M. Lazzari,Sonia M. N. Lazzarotto,Crisleide M. Panzavolta,Tiziana Iede,Edson T. Marques,Francisco de A. |
dc.subject.por.fl_str_mv |
Banded pine weevil Insecta molecular marker population genetics |
topic |
Banded pine weevil Insecta molecular marker population genetics |
description |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus. The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples may indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fst) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among populations points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them to respond differently to control measures. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0085-56262013005000036 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira De Entomologia |
publisher.none.fl_str_mv |
Sociedade Brasileira De Entomologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Entomologia v.57 n.4 2013 reponame:Revista brasileira de entomologia (Online) instname:Sociedade Brasileira De Entomologia (SBE) instacron:SBE |
instname_str |
Sociedade Brasileira De Entomologia (SBE) |
instacron_str |
SBE |
institution |
SBE |
reponame_str |
Revista brasileira de entomologia (Online) |
collection |
Revista brasileira de entomologia (Online) |
repository.name.fl_str_mv |
Revista brasileira de entomologia (Online) - Sociedade Brasileira De Entomologia (SBE) |
repository.mail.fl_str_mv |
||sbe@ufpr.br |
_version_ |
1752126459938340864 |