PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de entomologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014 |
Resumo: | The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among populations of An. cruzii varied from 0,0214 to 0,0673, suggesting that all individuals used in the analyses belong to a single species. The number of migrants per generation (Nm) was 4.3, showing the existence of gene flow among populations. The restriction profile of the ITS2, 5.8S and 28S gene regions was similar in all An. cruzii samples, whereas the results obtained by using HhaI and NruI are indicative that the individuals analyzed have nucleotide sequences distinct from those of An. cruzii samples from Peruíbe and Juquiazinho deposited in GenBank. |
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PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae)Anophelinaegenetic variabilityITS2molecular markersThe aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among populations of An. cruzii varied from 0,0214 to 0,0673, suggesting that all individuals used in the analyses belong to a single species. The number of migrants per generation (Nm) was 4.3, showing the existence of gene flow among populations. The restriction profile of the ITS2, 5.8S and 28S gene regions was similar in all An. cruzii samples, whereas the results obtained by using HhaI and NruI are indicative that the individuals analyzed have nucleotide sequences distinct from those of An. cruzii samples from Peruíbe and Juquiazinho deposited in GenBank.Sociedade Brasileira De Entomologia2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014Revista Brasileira de Entomologia v.50 n.3 2006reponame:Revista brasileira de entomologia (Online)instname:Sociedade Brasileira De Entomologia (SBE)instacron:SBE10.1590/S0085-56262006000300014info:eu-repo/semantics/openAccessCalado,DaniélaNavarro-Silva,Mario AntonioSallum,Maria Anice Murebeng2006-10-27T00:00:00Zoai:scielo:S0085-56262006000300014Revistahttp://www.rbentomologia.com/https://old.scielo.br/oai/scielo-oai.php||sbe@ufpr.br1806-96650085-5626opendoar:2006-10-27T00:00Revista brasileira de entomologia (Online) - Sociedade Brasileira De Entomologia (SBE)false |
dc.title.none.fl_str_mv |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
title |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
spellingShingle |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) Calado,Daniéla Anophelinae genetic variability ITS2 molecular markers |
title_short |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
title_full |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
title_fullStr |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
title_full_unstemmed |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
title_sort |
PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) |
author |
Calado,Daniéla |
author_facet |
Calado,Daniéla Navarro-Silva,Mario Antonio Sallum,Maria Anice Mureb |
author_role |
author |
author2 |
Navarro-Silva,Mario Antonio Sallum,Maria Anice Mureb |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Calado,Daniéla Navarro-Silva,Mario Antonio Sallum,Maria Anice Mureb |
dc.subject.por.fl_str_mv |
Anophelinae genetic variability ITS2 molecular markers |
topic |
Anophelinae genetic variability ITS2 molecular markers |
description |
The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among populations of An. cruzii varied from 0,0214 to 0,0673, suggesting that all individuals used in the analyses belong to a single species. The number of migrants per generation (Nm) was 4.3, showing the existence of gene flow among populations. The restriction profile of the ITS2, 5.8S and 28S gene regions was similar in all An. cruzii samples, whereas the results obtained by using HhaI and NruI are indicative that the individuals analyzed have nucleotide sequences distinct from those of An. cruzii samples from Peruíbe and Juquiazinho deposited in GenBank. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0085-56262006000300014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira De Entomologia |
publisher.none.fl_str_mv |
Sociedade Brasileira De Entomologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Entomologia v.50 n.3 2006 reponame:Revista brasileira de entomologia (Online) instname:Sociedade Brasileira De Entomologia (SBE) instacron:SBE |
instname_str |
Sociedade Brasileira De Entomologia (SBE) |
instacron_str |
SBE |
institution |
SBE |
reponame_str |
Revista brasileira de entomologia (Online) |
collection |
Revista brasileira de entomologia (Online) |
repository.name.fl_str_mv |
Revista brasileira de entomologia (Online) - Sociedade Brasileira De Entomologia (SBE) |
repository.mail.fl_str_mv |
||sbe@ufpr.br |
_version_ |
1752126457129205760 |