Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research on Biomedical Engineering (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402015000200085 |
Resumo: | Introduction Ultrasound biomicroscopy (UBM) is a technique for generating high-resolution images, with frequencies from 20 MHz to 100 MHz. For example, it has been used in animal research related to models of injury and diseases that mimic human conditions. With a three-dimensional ultrasound (3D) image system, an organ can be viewed at various angles and the volume estimated, contributing to an accurate diagnosis. This work refers to the generation of 3D-UBM images, employing a 35 MHz ultrasound system, from multiple two-dimensional (2D) images. Phantoms were used to validate the technique and to determine its reliability of volume measurements. Additionally, the technique was used to obtain 3D images of the rat gastrocnemius muscle. Methods Four different phantoms were used and ten acquisition sequences of 2D-images acquired for each one. Thereafter, 5 volume segmentations were performed for each acquisition sequence, resulting in 50 measured volumes for each phantom. The physical volumes of all phantoms were used to validate the technique based on the coefficient of variation (CV) and the intraclass correlation coefficient (ICC). Images of the gastrocnemius muscle were acquired and the partial volume quantified. Results The CV and ICC confirmed the reliability of volume measurements obtained by segmentation. Moreover, cross-sectional 2D images of rat hindlimb were obtained, allowing to identify the gastrocnemius muscle and to partially quantify the muscle volume from 3D images. Conclusion The results indicated that the technique is valid to generate 3D images and quantify the volume of a muscle compatible with the dimensions of a small animal. |
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Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemiusUltrasound biomicroscopy3D imagephantomgastrocnemius musclerat Introduction Ultrasound biomicroscopy (UBM) is a technique for generating high-resolution images, with frequencies from 20 MHz to 100 MHz. For example, it has been used in animal research related to models of injury and diseases that mimic human conditions. With a three-dimensional ultrasound (3D) image system, an organ can be viewed at various angles and the volume estimated, contributing to an accurate diagnosis. This work refers to the generation of 3D-UBM images, employing a 35 MHz ultrasound system, from multiple two-dimensional (2D) images. Phantoms were used to validate the technique and to determine its reliability of volume measurements. Additionally, the technique was used to obtain 3D images of the rat gastrocnemius muscle. Methods Four different phantoms were used and ten acquisition sequences of 2D-images acquired for each one. Thereafter, 5 volume segmentations were performed for each acquisition sequence, resulting in 50 measured volumes for each phantom. The physical volumes of all phantoms were used to validate the technique based on the coefficient of variation (CV) and the intraclass correlation coefficient (ICC). Images of the gastrocnemius muscle were acquired and the partial volume quantified. Results The CV and ICC confirmed the reliability of volume measurements obtained by segmentation. Moreover, cross-sectional 2D images of rat hindlimb were obtained, allowing to identify the gastrocnemius muscle and to partially quantify the muscle volume from 3D images. Conclusion The results indicated that the technique is valid to generate 3D images and quantify the volume of a muscle compatible with the dimensions of a small animal. Sociedade Brasileira de Engenharia Biomédica2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402015000200085Research on Biomedical Engineering v.31 n.2 2015reponame:Research on Biomedical Engineering (Online)instname:Sociedade Brasileira de Engenharia Biomédica (SBEB)instacron:SBEB10.1590/1517-3151.0209info:eu-repo/semantics/openAccessMartins,Natália Santos da FonsecaCarneiro,Luisa TinocoDantas,Hugo de MelloEsperança,CláudioMarroquim,Ricardo GuerraOliveira,Liliam Fernandes deMachado,João Carloseng2015-07-23T00:00:00Zoai:scielo:S2446-47402015000200085Revistahttp://www.rbejournal.org/https://old.scielo.br/oai/scielo-oai.php||rbe@rbejournal.org2446-47402446-4732opendoar:2015-07-23T00:00Research on Biomedical Engineering (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB)false |
dc.title.none.fl_str_mv |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
title |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
spellingShingle |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius Martins,Natália Santos da Fonseca Ultrasound biomicroscopy 3D image phantom gastrocnemius muscle rat |
title_short |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
title_full |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
title_fullStr |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
title_full_unstemmed |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
title_sort |
Generation of 3D ultrasound biomicroscopic images: technique validation and in vivo volumetric imaging of rat lateral gastrocnemius |
author |
Martins,Natália Santos da Fonseca |
author_facet |
Martins,Natália Santos da Fonseca Carneiro,Luisa Tinoco Dantas,Hugo de Mello Esperança,Cláudio Marroquim,Ricardo Guerra Oliveira,Liliam Fernandes de Machado,João Carlos |
author_role |
author |
author2 |
Carneiro,Luisa Tinoco Dantas,Hugo de Mello Esperança,Cláudio Marroquim,Ricardo Guerra Oliveira,Liliam Fernandes de Machado,João Carlos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Martins,Natália Santos da Fonseca Carneiro,Luisa Tinoco Dantas,Hugo de Mello Esperança,Cláudio Marroquim,Ricardo Guerra Oliveira,Liliam Fernandes de Machado,João Carlos |
dc.subject.por.fl_str_mv |
Ultrasound biomicroscopy 3D image phantom gastrocnemius muscle rat |
topic |
Ultrasound biomicroscopy 3D image phantom gastrocnemius muscle rat |
description |
Introduction Ultrasound biomicroscopy (UBM) is a technique for generating high-resolution images, with frequencies from 20 MHz to 100 MHz. For example, it has been used in animal research related to models of injury and diseases that mimic human conditions. With a three-dimensional ultrasound (3D) image system, an organ can be viewed at various angles and the volume estimated, contributing to an accurate diagnosis. This work refers to the generation of 3D-UBM images, employing a 35 MHz ultrasound system, from multiple two-dimensional (2D) images. Phantoms were used to validate the technique and to determine its reliability of volume measurements. Additionally, the technique was used to obtain 3D images of the rat gastrocnemius muscle. Methods Four different phantoms were used and ten acquisition sequences of 2D-images acquired for each one. Thereafter, 5 volume segmentations were performed for each acquisition sequence, resulting in 50 measured volumes for each phantom. The physical volumes of all phantoms were used to validate the technique based on the coefficient of variation (CV) and the intraclass correlation coefficient (ICC). Images of the gastrocnemius muscle were acquired and the partial volume quantified. Results The CV and ICC confirmed the reliability of volume measurements obtained by segmentation. Moreover, cross-sectional 2D images of rat hindlimb were obtained, allowing to identify the gastrocnemius muscle and to partially quantify the muscle volume from 3D images. Conclusion The results indicated that the technique is valid to generate 3D images and quantify the volume of a muscle compatible with the dimensions of a small animal. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402015000200085 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402015000200085 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1517-3151.0209 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Engenharia Biomédica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Engenharia Biomédica |
dc.source.none.fl_str_mv |
Research on Biomedical Engineering v.31 n.2 2015 reponame:Research on Biomedical Engineering (Online) instname:Sociedade Brasileira de Engenharia Biomédica (SBEB) instacron:SBEB |
instname_str |
Sociedade Brasileira de Engenharia Biomédica (SBEB) |
instacron_str |
SBEB |
institution |
SBEB |
reponame_str |
Research on Biomedical Engineering (Online) |
collection |
Research on Biomedical Engineering (Online) |
repository.name.fl_str_mv |
Research on Biomedical Engineering (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB) |
repository.mail.fl_str_mv |
||rbe@rbejournal.org |
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1752126288175300608 |