Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique

Detalhes bibliográficos
Autor(a) principal: Ríos,Melina Ocampo
Data de Publicação: 2007
Outros Autores: Fernández,Paula, Carmona,Marcelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Fitopatologia Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007
Resumo: Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each cultivar showing the presence of R. secalis. The use of specific primers was efficient in the detection of R. secalis in barley seeds in Argentina and could be used for routine diagnosis, epidemiology and seed transmission studies. This is the first report on the detection of R. secalis in barley seeds in Argentina.
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spelling Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction techniqueHordeum vulgarediagnosisPCRLeaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each cultivar showing the presence of R. secalis. The use of specific primers was efficient in the detection of R. secalis in barley seeds in Argentina and could be used for routine diagnosis, epidemiology and seed transmission studies. This is the first report on the detection of R. secalis in barley seeds in Argentina.Sociedade Brasileira de Fitopatologia2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007Fitopatologia Brasileira v.32 n.5 2007reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582007000500007info:eu-repo/semantics/openAccessRíos,Melina OcampoFernández,PaulaCarmona,Marceloeng2008-01-09T00:00:00Zoai:scielo:S0100-41582007000500007Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2008-01-09T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false
dc.title.none.fl_str_mv Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
title Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
spellingShingle Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
Ríos,Melina Ocampo
Hordeum vulgare
diagnosis
PCR
title_short Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
title_full Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
title_fullStr Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
title_full_unstemmed Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
title_sort Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
author Ríos,Melina Ocampo
author_facet Ríos,Melina Ocampo
Fernández,Paula
Carmona,Marcelo
author_role author
author2 Fernández,Paula
Carmona,Marcelo
author2_role author
author
dc.contributor.author.fl_str_mv Ríos,Melina Ocampo
Fernández,Paula
Carmona,Marcelo
dc.subject.por.fl_str_mv Hordeum vulgare
diagnosis
PCR
topic Hordeum vulgare
diagnosis
PCR
description Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each cultivar showing the presence of R. secalis. The use of specific primers was efficient in the detection of R. secalis in barley seeds in Argentina and could be used for routine diagnosis, epidemiology and seed transmission studies. This is the first report on the detection of R. secalis in barley seeds in Argentina.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-41582007000500007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Fitopatologia Brasileira v.32 n.5 2007
reponame:Fitopatologia Brasileira
instname:Sociedade Brasileira de Fitopatologia (SBF)
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia (SBF)
instacron_str SBF
institution SBF
reponame_str Fitopatologia Brasileira
collection Fitopatologia Brasileira
repository.name.fl_str_mv Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)
repository.mail.fl_str_mv ||sbf-revista@ufla.br
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