Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil

Detalhes bibliográficos
Autor(a) principal: AMBROZEVICIUS,LUCIANA P.
Data de Publicação: 2002
Outros Autores: CALEGARIO,RENATA F., FONTES,ELIZABETH P.B., CARVALHO,MURILO G. DE, ZERBINI,F. MURILO
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Fitopatologia Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400006
Resumo: The genetic diversity of begomovirus isolates from tomato (Lycopersicon esculentum) fields in the Southeastern region of Brazil was analyzed by direct sequencing of PCR fragments amplified by using universal oligonucleotides for the begomovirus DNA-A, and subsequent computer-aided phylogenetic analysis. Samples of tomato plants and associated weeds showing typical symptoms of virus infection were collected at seven locations in the states of Minas Gerais, Espírito Santo and Rio de Janeiro. A total of 137 out of 369 samples were infected with a begomovirus based on PCR analysis. Phylogenetic analysis indicated a high degree of genetic diversity among begomoviruses infecting tomatoes in the sampled area. One species (Tomato chlorotic mottle virus, TCMV) occurs predominantly in Minas Gerais, whereas in Rio de Janeiro and Espírito Santo a distinct species, not yet fully characterized, predominates. Phylogenetic analysis further indicates the presence of an additional four possible new species. This high degree of genetic diversity suggests a recent transfer of indigenous begomovirus from wild hosts into tomatoes. The close phylogenetic relationship verified between begomovirus infecting tomato and associated weeds favors this hypothesis.
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spelling Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern BrazilGeminiviridaephylogenetic relationshipsTomato chlorotic mottle virusThe genetic diversity of begomovirus isolates from tomato (Lycopersicon esculentum) fields in the Southeastern region of Brazil was analyzed by direct sequencing of PCR fragments amplified by using universal oligonucleotides for the begomovirus DNA-A, and subsequent computer-aided phylogenetic analysis. Samples of tomato plants and associated weeds showing typical symptoms of virus infection were collected at seven locations in the states of Minas Gerais, Espírito Santo and Rio de Janeiro. A total of 137 out of 369 samples were infected with a begomovirus based on PCR analysis. Phylogenetic analysis indicated a high degree of genetic diversity among begomoviruses infecting tomatoes in the sampled area. One species (Tomato chlorotic mottle virus, TCMV) occurs predominantly in Minas Gerais, whereas in Rio de Janeiro and Espírito Santo a distinct species, not yet fully characterized, predominates. Phylogenetic analysis further indicates the presence of an additional four possible new species. This high degree of genetic diversity suggests a recent transfer of indigenous begomovirus from wild hosts into tomatoes. The close phylogenetic relationship verified between begomovirus infecting tomato and associated weeds favors this hypothesis.Sociedade Brasileira de Fitopatologia2002-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400006Fitopatologia Brasileira v.27 n.4 2002reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582002000400006info:eu-repo/semantics/openAccessAMBROZEVICIUS,LUCIANA P.CALEGARIO,RENATA F.FONTES,ELIZABETH P.B.CARVALHO,MURILO G. DEZERBINI,F. MURILOeng2002-10-01T00:00:00Zoai:scielo:S0100-41582002000400006Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2002-10-01T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false
dc.title.none.fl_str_mv Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
title Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
spellingShingle Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
AMBROZEVICIUS,LUCIANA P.
Geminiviridae
phylogenetic relationships
Tomato chlorotic mottle virus
title_short Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
title_full Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
title_fullStr Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
title_full_unstemmed Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
title_sort Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil
author AMBROZEVICIUS,LUCIANA P.
author_facet AMBROZEVICIUS,LUCIANA P.
CALEGARIO,RENATA F.
FONTES,ELIZABETH P.B.
CARVALHO,MURILO G. DE
ZERBINI,F. MURILO
author_role author
author2 CALEGARIO,RENATA F.
FONTES,ELIZABETH P.B.
CARVALHO,MURILO G. DE
ZERBINI,F. MURILO
author2_role author
author
author
author
dc.contributor.author.fl_str_mv AMBROZEVICIUS,LUCIANA P.
CALEGARIO,RENATA F.
FONTES,ELIZABETH P.B.
CARVALHO,MURILO G. DE
ZERBINI,F. MURILO
dc.subject.por.fl_str_mv Geminiviridae
phylogenetic relationships
Tomato chlorotic mottle virus
topic Geminiviridae
phylogenetic relationships
Tomato chlorotic mottle virus
description The genetic diversity of begomovirus isolates from tomato (Lycopersicon esculentum) fields in the Southeastern region of Brazil was analyzed by direct sequencing of PCR fragments amplified by using universal oligonucleotides for the begomovirus DNA-A, and subsequent computer-aided phylogenetic analysis. Samples of tomato plants and associated weeds showing typical symptoms of virus infection were collected at seven locations in the states of Minas Gerais, Espírito Santo and Rio de Janeiro. A total of 137 out of 369 samples were infected with a begomovirus based on PCR analysis. Phylogenetic analysis indicated a high degree of genetic diversity among begomoviruses infecting tomatoes in the sampled area. One species (Tomato chlorotic mottle virus, TCMV) occurs predominantly in Minas Gerais, whereas in Rio de Janeiro and Espírito Santo a distinct species, not yet fully characterized, predominates. Phylogenetic analysis further indicates the presence of an additional four possible new species. This high degree of genetic diversity suggests a recent transfer of indigenous begomovirus from wild hosts into tomatoes. The close phylogenetic relationship verified between begomovirus infecting tomato and associated weeds favors this hypothesis.
publishDate 2002
dc.date.none.fl_str_mv 2002-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-41582002000400006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Fitopatologia Brasileira v.27 n.4 2002
reponame:Fitopatologia Brasileira
instname:Sociedade Brasileira de Fitopatologia (SBF)
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia (SBF)
instacron_str SBF
institution SBF
reponame_str Fitopatologia Brasileira
collection Fitopatologia Brasileira
repository.name.fl_str_mv Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)
repository.mail.fl_str_mv ||sbf-revista@ufla.br
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