Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Fitopatologia Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100008 |
Resumo: | Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected. |
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Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprintingdiversitygrapevine bacterial cankerVitis viniferarepetitive DNABacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.Sociedade Brasileira de Fitopatologia2005-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100008Fitopatologia Brasileira v.30 n.1 2005reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582005000100008info:eu-repo/semantics/openAccessTrindade,Loiselene C.Lima,Mirtes F.Ferreira,Marisa A. S. V.eng2005-03-21T00:00:00Zoai:scielo:S0100-41582005000100008Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2005-03-21T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false |
dc.title.none.fl_str_mv |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
title |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
spellingShingle |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting Trindade,Loiselene C. diversity grapevine bacterial canker Vitis vinifera repetitive DNA |
title_short |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
title_full |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
title_fullStr |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
title_full_unstemmed |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
title_sort |
Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting |
author |
Trindade,Loiselene C. |
author_facet |
Trindade,Loiselene C. Lima,Mirtes F. Ferreira,Marisa A. S. V. |
author_role |
author |
author2 |
Lima,Mirtes F. Ferreira,Marisa A. S. V. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Trindade,Loiselene C. Lima,Mirtes F. Ferreira,Marisa A. S. V. |
dc.subject.por.fl_str_mv |
diversity grapevine bacterial canker Vitis vinifera repetitive DNA |
topic |
diversity grapevine bacterial canker Vitis vinifera repetitive DNA |
description |
Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-41582005000100008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Fitopatologia Brasileira v.30 n.1 2005 reponame:Fitopatologia Brasileira instname:Sociedade Brasileira de Fitopatologia (SBF) instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia (SBF) |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Fitopatologia Brasileira |
collection |
Fitopatologia Brasileira |
repository.name.fl_str_mv |
Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF) |
repository.mail.fl_str_mv |
||sbf-revista@ufla.br |
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1754734650207502336 |