Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus

Detalhes bibliográficos
Autor(a) principal: Souza Júnior,Manoel T.
Data de Publicação: 2005
Outros Autores: Nickel,Osmar, Gonsalves,Dennis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Fitopatologia Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004
Resumo: Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.
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spelling Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virusCarica papayapotyvirusresistancebreedingvirusTranslatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.Sociedade Brasileira de Fitopatologia2005-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004Fitopatologia Brasileira v.30 n.4 2005reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582005000400004info:eu-repo/semantics/openAccessSouza Júnior,Manoel T.Nickel,OsmarGonsalves,Denniseng2005-10-17T00:00:00Zoai:scielo:S0100-41582005000400004Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2005-10-17T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false
dc.title.none.fl_str_mv Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
title Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
spellingShingle Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
Souza Júnior,Manoel T.
Carica papaya
potyvirus
resistance
breeding
virus
title_short Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
title_full Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
title_fullStr Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
title_full_unstemmed Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
title_sort Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
author Souza Júnior,Manoel T.
author_facet Souza Júnior,Manoel T.
Nickel,Osmar
Gonsalves,Dennis
author_role author
author2 Nickel,Osmar
Gonsalves,Dennis
author2_role author
author
dc.contributor.author.fl_str_mv Souza Júnior,Manoel T.
Nickel,Osmar
Gonsalves,Dennis
dc.subject.por.fl_str_mv Carica papaya
potyvirus
resistance
breeding
virus
topic Carica papaya
potyvirus
resistance
breeding
virus
description Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.
publishDate 2005
dc.date.none.fl_str_mv 2005-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-41582005000400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Fitopatologia Brasileira v.30 n.4 2005
reponame:Fitopatologia Brasileira
instname:Sociedade Brasileira de Fitopatologia (SBF)
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia (SBF)
instacron_str SBF
institution SBF
reponame_str Fitopatologia Brasileira
collection Fitopatologia Brasileira
repository.name.fl_str_mv Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)
repository.mail.fl_str_mv ||sbf-revista@ufla.br
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