Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Fitopatologia Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005 |
Resumo: | A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR. |
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Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazilnucleotide sequenceElisahost rangeA virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.Sociedade Brasileira de Fitopatologia2005-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005Fitopatologia Brasileira v.30 n.4 2005reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582005000400005info:eu-repo/semantics/openAccessAlmeida,Álvaro M. R.Sakai,JunichiHanada,KaoruOliveira,Thalita G.Belintani,PriscilaKitajima,Elliot W.Souto,Eliezer R.Novaes,Tanara G. deNora,Paulo S.eng2005-10-17T00:00:00Zoai:scielo:S0100-41582005000400005Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2005-10-17T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false |
dc.title.none.fl_str_mv |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
spellingShingle |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil Almeida,Álvaro M. R. nucleotide sequence Elisa host range |
title_short |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_full |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_fullStr |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_full_unstemmed |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_sort |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
author |
Almeida,Álvaro M. R. |
author_facet |
Almeida,Álvaro M. R. Sakai,Junichi Hanada,Kaoru Oliveira,Thalita G. Belintani,Priscila Kitajima,Elliot W. Souto,Eliezer R. Novaes,Tanara G. de Nora,Paulo S. |
author_role |
author |
author2 |
Sakai,Junichi Hanada,Kaoru Oliveira,Thalita G. Belintani,Priscila Kitajima,Elliot W. Souto,Eliezer R. Novaes,Tanara G. de Nora,Paulo S. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Almeida,Álvaro M. R. Sakai,Junichi Hanada,Kaoru Oliveira,Thalita G. Belintani,Priscila Kitajima,Elliot W. Souto,Eliezer R. Novaes,Tanara G. de Nora,Paulo S. |
dc.subject.por.fl_str_mv |
nucleotide sequence Elisa host range |
topic |
nucleotide sequence Elisa host range |
description |
A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-41582005000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Fitopatologia Brasileira v.30 n.4 2005 reponame:Fitopatologia Brasileira instname:Sociedade Brasileira de Fitopatologia (SBF) instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia (SBF) |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Fitopatologia Brasileira |
collection |
Fitopatologia Brasileira |
repository.name.fl_str_mv |
Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF) |
repository.mail.fl_str_mv |
||sbf-revista@ufla.br |
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1754734650284048384 |