Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Beserra Jr.,José Evando A.
Data de Publicação: 2011
Outros Autores: Andrade,Eduardo C., Camarço,Rosa F.R. Araújo, Nascimento,Aline K.Q., Lima,José Albérsio A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200009
Resumo: The comovirus Cowpea severe mosaic virus (CPSMV) causes a disease in cowpea (Vigna unguiculata) but, despite its importance, there are no studies on the genetic diversity of viral populations. We have determined the nucleotide sequences of part of the coat protein (CP) gene of six Brazilian isolates of CPSMV. Genomic fragments (521 nucleotides) were RT-PCR amplified, cloned, and their sequences were compared with each other and with other comoviruses. Sequence comparisons indicated a high degree of conservation for the CP gene, with 92-100% nucleotide and 97-100% amino acid sequence identity among the isolates. There was no correlation between geographical origin and sequence identity or phylogeny among the isolates.
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spelling Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern BrazilComovirusVigna unguiculataCPSMVsequence diversityThe comovirus Cowpea severe mosaic virus (CPSMV) causes a disease in cowpea (Vigna unguiculata) but, despite its importance, there are no studies on the genetic diversity of viral populations. We have determined the nucleotide sequences of part of the coat protein (CP) gene of six Brazilian isolates of CPSMV. Genomic fragments (521 nucleotides) were RT-PCR amplified, cloned, and their sequences were compared with each other and with other comoviruses. Sequence comparisons indicated a high degree of conservation for the CP gene, with 92-100% nucleotide and 97-100% amino acid sequence identity among the isolates. There was no correlation between geographical origin and sequence identity or phylogeny among the isolates.Sociedade Brasileira de Fitopatologia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200009Tropical Plant Pathology v.36 n.2 2011reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762011000200009info:eu-repo/semantics/openAccessBeserra Jr.,José Evando A.Andrade,Eduardo C.Camarço,Rosa F.R. AraújoNascimento,Aline K.Q.Lima,José Albérsio A.eng2011-06-17T00:00:00Zoai:scielo:S1982-56762011000200009Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-06-17T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
title Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
spellingShingle Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
Beserra Jr.,José Evando A.
Comovirus
Vigna unguiculata
CPSMV
sequence diversity
title_short Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
title_full Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
title_fullStr Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
title_full_unstemmed Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
title_sort Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil
author Beserra Jr.,José Evando A.
author_facet Beserra Jr.,José Evando A.
Andrade,Eduardo C.
Camarço,Rosa F.R. Araújo
Nascimento,Aline K.Q.
Lima,José Albérsio A.
author_role author
author2 Andrade,Eduardo C.
Camarço,Rosa F.R. Araújo
Nascimento,Aline K.Q.
Lima,José Albérsio A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Beserra Jr.,José Evando A.
Andrade,Eduardo C.
Camarço,Rosa F.R. Araújo
Nascimento,Aline K.Q.
Lima,José Albérsio A.
dc.subject.por.fl_str_mv Comovirus
Vigna unguiculata
CPSMV
sequence diversity
topic Comovirus
Vigna unguiculata
CPSMV
sequence diversity
description The comovirus Cowpea severe mosaic virus (CPSMV) causes a disease in cowpea (Vigna unguiculata) but, despite its importance, there are no studies on the genetic diversity of viral populations. We have determined the nucleotide sequences of part of the coat protein (CP) gene of six Brazilian isolates of CPSMV. Genomic fragments (521 nucleotides) were RT-PCR amplified, cloned, and their sequences were compared with each other and with other comoviruses. Sequence comparisons indicated a high degree of conservation for the CP gene, with 92-100% nucleotide and 97-100% amino acid sequence identity among the isolates. There was no correlation between geographical origin and sequence identity or phylogeny among the isolates.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762011000200009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.36 n.2 2011
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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