Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)

Detalhes bibliográficos
Autor(a) principal: González-Aguilera,Jorge
Data de Publicação: 2012
Outros Autores: Tavares,Sheila S., Sobrinho,Roberto R., Xavier,César A.D., Dueñas-Hurtado,Francisco, Lara-Rodrigues,Regla M., Silva,Derly J.H. da, Zerbini,F. Murilo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007
Resumo: Begomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, in Nov/2009 and Dec/2010. Viral genomes were amplified, cloned and sequenced. A total of 36 DNA-A components were obtained. Sequence comparisons indicated the presence of a single begomovirus, Tomato severe rugose virus (ToSRV), with pairwise identities between isolates ranging from 97.3 to 100%. Subdivision tests indicated the existence of a single population. The analysis of variability descriptors indicated that the ToSRV population has a genetic variability similar to other begomovirus populations described in Brazil infecting tomato. Neutrality tests suggested the occurrence of purifying selection acting upon the population. Recombination analysis identified recombination events with begomoviruses from the weed species Sida micrantha. The wide distribution of ToSRV in the field and the detection of recombination indicate that continuous monitoring of viral populations in the field will be required to enable an efficient resistance-based control strategy for begomoviruses.
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spelling Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)geminivirusgenetic variabilityrecombinationBegomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, in Nov/2009 and Dec/2010. Viral genomes were amplified, cloned and sequenced. A total of 36 DNA-A components were obtained. Sequence comparisons indicated the presence of a single begomovirus, Tomato severe rugose virus (ToSRV), with pairwise identities between isolates ranging from 97.3 to 100%. Subdivision tests indicated the existence of a single population. The analysis of variability descriptors indicated that the ToSRV population has a genetic variability similar to other begomovirus populations described in Brazil infecting tomato. Neutrality tests suggested the occurrence of purifying selection acting upon the population. Recombination analysis identified recombination events with begomoviruses from the weed species Sida micrantha. The wide distribution of ToSRV in the field and the detection of recombination indicate that continuous monitoring of viral populations in the field will be required to enable an efficient resistance-based control strategy for begomoviruses.Sociedade Brasileira de Fitopatologia2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007Tropical Plant Pathology v.37 n.5 2012reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762012000500007info:eu-repo/semantics/openAccessGonzález-Aguilera,JorgeTavares,Sheila S.Sobrinho,Roberto R.Xavier,César A.D.Dueñas-Hurtado,FranciscoLara-Rodrigues,Regla M.Silva,Derly J.H. daZerbini,F. Muriloeng2012-10-31T00:00:00Zoai:scielo:S1982-56762012000500007Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2012-10-31T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
title Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
spellingShingle Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
González-Aguilera,Jorge
geminivirus
genetic variability
recombination
title_short Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
title_full Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
title_fullStr Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
title_full_unstemmed Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
title_sort Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
author González-Aguilera,Jorge
author_facet González-Aguilera,Jorge
Tavares,Sheila S.
Sobrinho,Roberto R.
Xavier,César A.D.
Dueñas-Hurtado,Francisco
Lara-Rodrigues,Regla M.
Silva,Derly J.H. da
Zerbini,F. Murilo
author_role author
author2 Tavares,Sheila S.
Sobrinho,Roberto R.
Xavier,César A.D.
Dueñas-Hurtado,Francisco
Lara-Rodrigues,Regla M.
Silva,Derly J.H. da
Zerbini,F. Murilo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv González-Aguilera,Jorge
Tavares,Sheila S.
Sobrinho,Roberto R.
Xavier,César A.D.
Dueñas-Hurtado,Francisco
Lara-Rodrigues,Regla M.
Silva,Derly J.H. da
Zerbini,F. Murilo
dc.subject.por.fl_str_mv geminivirus
genetic variability
recombination
topic geminivirus
genetic variability
recombination
description Begomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, in Nov/2009 and Dec/2010. Viral genomes were amplified, cloned and sequenced. A total of 36 DNA-A components were obtained. Sequence comparisons indicated the presence of a single begomovirus, Tomato severe rugose virus (ToSRV), with pairwise identities between isolates ranging from 97.3 to 100%. Subdivision tests indicated the existence of a single population. The analysis of variability descriptors indicated that the ToSRV population has a genetic variability similar to other begomovirus populations described in Brazil infecting tomato. Neutrality tests suggested the occurrence of purifying selection acting upon the population. Recombination analysis identified recombination events with begomoviruses from the weed species Sida micrantha. The wide distribution of ToSRV in the field and the detection of recombination indicate that continuous monitoring of viral populations in the field will be required to enable an efficient resistance-based control strategy for begomoviruses.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762012000500007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.37 n.5 2012
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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