Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007 |
Resumo: | Begomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, in Nov/2009 and Dec/2010. Viral genomes were amplified, cloned and sequenced. A total of 36 DNA-A components were obtained. Sequence comparisons indicated the presence of a single begomovirus, Tomato severe rugose virus (ToSRV), with pairwise identities between isolates ranging from 97.3 to 100%. Subdivision tests indicated the existence of a single population. The analysis of variability descriptors indicated that the ToSRV population has a genetic variability similar to other begomovirus populations described in Brazil infecting tomato. Neutrality tests suggested the occurrence of purifying selection acting upon the population. Recombination analysis identified recombination events with begomoviruses from the weed species Sida micrantha. The wide distribution of ToSRV in the field and the detection of recombination indicate that continuous monitoring of viral populations in the field will be required to enable an efficient resistance-based control strategy for begomoviruses. |
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Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)geminivirusgenetic variabilityrecombinationBegomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, in Nov/2009 and Dec/2010. Viral genomes were amplified, cloned and sequenced. A total of 36 DNA-A components were obtained. Sequence comparisons indicated the presence of a single begomovirus, Tomato severe rugose virus (ToSRV), with pairwise identities between isolates ranging from 97.3 to 100%. Subdivision tests indicated the existence of a single population. The analysis of variability descriptors indicated that the ToSRV population has a genetic variability similar to other begomovirus populations described in Brazil infecting tomato. Neutrality tests suggested the occurrence of purifying selection acting upon the population. Recombination analysis identified recombination events with begomoviruses from the weed species Sida micrantha. The wide distribution of ToSRV in the field and the detection of recombination indicate that continuous monitoring of viral populations in the field will be required to enable an efficient resistance-based control strategy for begomoviruses.Sociedade Brasileira de Fitopatologia2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007Tropical Plant Pathology v.37 n.5 2012reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762012000500007info:eu-repo/semantics/openAccessGonzález-Aguilera,JorgeTavares,Sheila S.Sobrinho,Roberto R.Xavier,César A.D.Dueñas-Hurtado,FranciscoLara-Rodrigues,Regla M.Silva,Derly J.H. daZerbini,F. Muriloeng2012-10-31T00:00:00Zoai:scielo:S1982-56762012000500007Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2012-10-31T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
title |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
spellingShingle |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) González-Aguilera,Jorge geminivirus genetic variability recombination |
title_short |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
title_full |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
title_fullStr |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
title_full_unstemmed |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
title_sort |
Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV) |
author |
González-Aguilera,Jorge |
author_facet |
González-Aguilera,Jorge Tavares,Sheila S. Sobrinho,Roberto R. Xavier,César A.D. Dueñas-Hurtado,Francisco Lara-Rodrigues,Regla M. Silva,Derly J.H. da Zerbini,F. Murilo |
author_role |
author |
author2 |
Tavares,Sheila S. Sobrinho,Roberto R. Xavier,César A.D. Dueñas-Hurtado,Francisco Lara-Rodrigues,Regla M. Silva,Derly J.H. da Zerbini,F. Murilo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
González-Aguilera,Jorge Tavares,Sheila S. Sobrinho,Roberto R. Xavier,César A.D. Dueñas-Hurtado,Francisco Lara-Rodrigues,Regla M. Silva,Derly J.H. da Zerbini,F. Murilo |
dc.subject.por.fl_str_mv |
geminivirus genetic variability recombination |
topic |
geminivirus genetic variability recombination |
description |
Begomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, in Nov/2009 and Dec/2010. Viral genomes were amplified, cloned and sequenced. A total of 36 DNA-A components were obtained. Sequence comparisons indicated the presence of a single begomovirus, Tomato severe rugose virus (ToSRV), with pairwise identities between isolates ranging from 97.3 to 100%. Subdivision tests indicated the existence of a single population. The analysis of variability descriptors indicated that the ToSRV population has a genetic variability similar to other begomovirus populations described in Brazil infecting tomato. Neutrality tests suggested the occurrence of purifying selection acting upon the population. Recombination analysis identified recombination events with begomoviruses from the weed species Sida micrantha. The wide distribution of ToSRV in the field and the detection of recombination indicate that continuous monitoring of viral populations in the field will be required to enable an efficient resistance-based control strategy for begomoviruses. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000500007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762012000500007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.37 n.5 2012 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824585896787968 |