Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000600001 |
Resumo: | Web blight is one of the main diseases that affects bean (Phaseolus vulgaris) cultivation. It infects diverse organs at any growth stage of the plant and can be present at different altitudes in a humid tropical climate. The causal agent of this disease is Thanatephorus cucumeris in its sexual stage and Rhizoctonia solani in the anamorph. The objective of this investigation was to characterize molecular isolates of R. solani obtained from bean plants from diverse production regions in Costa Rica and determine their virulence. Fifty-one samples of symptomatic bean plants were collected using a global positioning system. Virulence was evaluated using the detached leaf technique. Isolates were identified using AG 1-IA, AG 1-IB, AG 1-IC, AG 1-ID, AG 2-2, AG 2-2IIIB, AG 2-2IV and AG 4 molecular markers. ITS sequences were obtained and analyzed with BLAST, aligned, and a phylogenetic tree was constructed. A high degree of virulence and genetic variability between isolates was identified and the anastomosis subgroups of isolates were independent of their geographical origin. |
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Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common beanPhaseolus vulgarisanastomosis group (AG)genetic variabilitymolecular characterizationsclerotium sizevirulenceWeb blight is one of the main diseases that affects bean (Phaseolus vulgaris) cultivation. It infects diverse organs at any growth stage of the plant and can be present at different altitudes in a humid tropical climate. The causal agent of this disease is Thanatephorus cucumeris in its sexual stage and Rhizoctonia solani in the anamorph. The objective of this investigation was to characterize molecular isolates of R. solani obtained from bean plants from diverse production regions in Costa Rica and determine their virulence. Fifty-one samples of symptomatic bean plants were collected using a global positioning system. Virulence was evaluated using the detached leaf technique. Isolates were identified using AG 1-IA, AG 1-IB, AG 1-IC, AG 1-ID, AG 2-2, AG 2-2IIIB, AG 2-2IV and AG 4 molecular markers. ITS sequences were obtained and analyzed with BLAST, aligned, and a phylogenetic tree was constructed. A high degree of virulence and genetic variability between isolates was identified and the anastomosis subgroups of isolates were independent of their geographical origin.Sociedade Brasileira de Fitopatologia2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000600001Tropical Plant Pathology v.38 n.6 2013reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762013000600001info:eu-repo/semantics/openAccessMora-Umaña,FloribethBarboza,NataliaAlvarado,RicardoVásquez,MarcelaGodoy-Lutz,GracielaSteadman,James R.Ramírez,Pilareng2013-12-17T00:00:00Zoai:scielo:S1982-56762013000600001Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2013-12-17T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
title |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
spellingShingle |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean Mora-Umaña,Floribeth Phaseolus vulgaris anastomosis group (AG) genetic variability molecular characterization sclerotium size virulence |
title_short |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
title_full |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
title_fullStr |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
title_full_unstemmed |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
title_sort |
Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean |
author |
Mora-Umaña,Floribeth |
author_facet |
Mora-Umaña,Floribeth Barboza,Natalia Alvarado,Ricardo Vásquez,Marcela Godoy-Lutz,Graciela Steadman,James R. Ramírez,Pilar |
author_role |
author |
author2 |
Barboza,Natalia Alvarado,Ricardo Vásquez,Marcela Godoy-Lutz,Graciela Steadman,James R. Ramírez,Pilar |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Mora-Umaña,Floribeth Barboza,Natalia Alvarado,Ricardo Vásquez,Marcela Godoy-Lutz,Graciela Steadman,James R. Ramírez,Pilar |
dc.subject.por.fl_str_mv |
Phaseolus vulgaris anastomosis group (AG) genetic variability molecular characterization sclerotium size virulence |
topic |
Phaseolus vulgaris anastomosis group (AG) genetic variability molecular characterization sclerotium size virulence |
description |
Web blight is one of the main diseases that affects bean (Phaseolus vulgaris) cultivation. It infects diverse organs at any growth stage of the plant and can be present at different altitudes in a humid tropical climate. The causal agent of this disease is Thanatephorus cucumeris in its sexual stage and Rhizoctonia solani in the anamorph. The objective of this investigation was to characterize molecular isolates of R. solani obtained from bean plants from diverse production regions in Costa Rica and determine their virulence. Fifty-one samples of symptomatic bean plants were collected using a global positioning system. Virulence was evaluated using the detached leaf technique. Isolates were identified using AG 1-IA, AG 1-IB, AG 1-IC, AG 1-ID, AG 2-2, AG 2-2IIIB, AG 2-2IV and AG 4 molecular markers. ITS sequences were obtained and analyzed with BLAST, aligned, and a phylogenetic tree was constructed. A high degree of virulence and genetic variability between isolates was identified and the anastomosis subgroups of isolates were independent of their geographical origin. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000600001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000600001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762013000600001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.38 n.6 2013 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824586276372480 |