First report of recombination in Potato yellow vein virus (PYVV) in Colombia
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300007 |
Resumo: | Potato yellow vein virus (PYVV) is currently one of the most important viruses that infects potatoes in Colombia and other Andean countries, causing losses in the production of tubers ranging from 25% to 50%. This study analyzed the genetic variability of different viral isolates collected in the department of Nariño, Colombia, through bioinformatics analysis of the sequences of three genes encoding the capsid protein (CP), the heat-shock protein 70 (Hsp70) and the minor capsid protein (CPm). We found that CPm is the gene that shows greater diversity, with higher values of nucleotide substitution and evidence of recombination. Based on an analysis of the haplotype map using nucleotide sequences of the CPm, we propose a model of putative recombination in this genomic region. The non-recombinant segments are supported by the results of the program GARD (Genetic Algorithm for Recombination Detection), phylogenetic trees and the paired values of genetic distances of each non-recombinant segments. The model clearly shows that the amino region of the CPm is prone to recombination. To our knowledge, this is the first report of genetic recombination as an evolutionary strategy in the CPm of PYVV. |
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First report of recombination in Potato yellow vein virus (PYVV) in ColombiaCrinivirusSolanum tuberosumgenetic variabilityminor coat proteinPotato yellow vein virus (PYVV) is currently one of the most important viruses that infects potatoes in Colombia and other Andean countries, causing losses in the production of tubers ranging from 25% to 50%. This study analyzed the genetic variability of different viral isolates collected in the department of Nariño, Colombia, through bioinformatics analysis of the sequences of three genes encoding the capsid protein (CP), the heat-shock protein 70 (Hsp70) and the minor capsid protein (CPm). We found that CPm is the gene that shows greater diversity, with higher values of nucleotide substitution and evidence of recombination. Based on an analysis of the haplotype map using nucleotide sequences of the CPm, we propose a model of putative recombination in this genomic region. The non-recombinant segments are supported by the results of the program GARD (Genetic Algorithm for Recombination Detection), phylogenetic trees and the paired values of genetic distances of each non-recombinant segments. The model clearly shows that the amino region of the CPm is prone to recombination. To our knowledge, this is the first report of genetic recombination as an evolutionary strategy in the CPm of PYVV.Sociedade Brasileira de Fitopatologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300007Tropical Plant Pathology v.39 n.3 2014reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762014000300007info:eu-repo/semantics/openAccessChaves-Bedoya,GiovanniCubillos,KarenGuzmán-Barney,Mónicaeng2014-06-03T00:00:00Zoai:scielo:S1982-56762014000300007Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2014-06-03T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
title |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
spellingShingle |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia Chaves-Bedoya,Giovanni Crinivirus Solanum tuberosum genetic variability minor coat protein |
title_short |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
title_full |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
title_fullStr |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
title_full_unstemmed |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
title_sort |
First report of recombination in Potato yellow vein virus (PYVV) in Colombia |
author |
Chaves-Bedoya,Giovanni |
author_facet |
Chaves-Bedoya,Giovanni Cubillos,Karen Guzmán-Barney,Mónica |
author_role |
author |
author2 |
Cubillos,Karen Guzmán-Barney,Mónica |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Chaves-Bedoya,Giovanni Cubillos,Karen Guzmán-Barney,Mónica |
dc.subject.por.fl_str_mv |
Crinivirus Solanum tuberosum genetic variability minor coat protein |
topic |
Crinivirus Solanum tuberosum genetic variability minor coat protein |
description |
Potato yellow vein virus (PYVV) is currently one of the most important viruses that infects potatoes in Colombia and other Andean countries, causing losses in the production of tubers ranging from 25% to 50%. This study analyzed the genetic variability of different viral isolates collected in the department of Nariño, Colombia, through bioinformatics analysis of the sequences of three genes encoding the capsid protein (CP), the heat-shock protein 70 (Hsp70) and the minor capsid protein (CPm). We found that CPm is the gene that shows greater diversity, with higher values of nucleotide substitution and evidence of recombination. Based on an analysis of the haplotype map using nucleotide sequences of the CPm, we propose a model of putative recombination in this genomic region. The non-recombinant segments are supported by the results of the program GARD (Genetic Algorithm for Recombination Detection), phylogenetic trees and the paired values of genetic distances of each non-recombinant segments. The model clearly shows that the amino region of the CPm is prone to recombination. To our knowledge, this is the first report of genetic recombination as an evolutionary strategy in the CPm of PYVV. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762014000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.39 n.3 2014 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824586377035776 |