Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000400005 |
Resumo: | The use of in vitro mass multiplication of nematodes under axenic conditions allows intensification of studies on taxonomy, biology, epidemiology and control of these pathogens. In the present study, two methods were compared for in vitro multiplication of two species of plant-parasitic nematodes, Radopholus similis and Pratylenchus brachyurus in 20, 40 and 60 day-periods. Both techniques involved the use of carrot cylinders placed in jars with lids, with water-agar in the bottom of the flasks (WA+) or without (WA-). In WA- the nematodes were treated with a 1% solution of ampicillin whilst for WA+ the axenization was carried out with a solution of mercuric chloride (0.01%), and streptomycin sulfate 0.02%. The WA+ condition was the most favorable for multiplication of both species of nematodes, resulting in a population increase of about 280 times for R. similis and 226 times for P. brachyurus in relation to the founding population of 25 individuals. For WA- a population increase of only five times after 60 days for R. similis and a duplication of the population after 60 days for P. brachyurus was obtained. Overall, the largest amount of nematodes occurred at 60 days after inoculation. |
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Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylindersBurrowing nematodein vitro multiplicationroot lesion nematodeThe use of in vitro mass multiplication of nematodes under axenic conditions allows intensification of studies on taxonomy, biology, epidemiology and control of these pathogens. In the present study, two methods were compared for in vitro multiplication of two species of plant-parasitic nematodes, Radopholus similis and Pratylenchus brachyurus in 20, 40 and 60 day-periods. Both techniques involved the use of carrot cylinders placed in jars with lids, with water-agar in the bottom of the flasks (WA+) or without (WA-). In WA- the nematodes were treated with a 1% solution of ampicillin whilst for WA+ the axenization was carried out with a solution of mercuric chloride (0.01%), and streptomycin sulfate 0.02%. The WA+ condition was the most favorable for multiplication of both species of nematodes, resulting in a population increase of about 280 times for R. similis and 226 times for P. brachyurus in relation to the founding population of 25 individuals. For WA- a population increase of only five times after 60 days for R. similis and a duplication of the population after 60 days for P. brachyurus was obtained. Overall, the largest amount of nematodes occurred at 60 days after inoculation.Sociedade Brasileira de Fitopatologia2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000400005Tropical Plant Pathology v.37 n.4 2012reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762012000400005info:eu-repo/semantics/openAccessSantos,Jansen R.P.Andrade,Ednalva P.Costa,Dilson C.Gonzaga,VilmarCares,Juvenil E.eng2012-08-10T00:00:00Zoai:scielo:S1982-56762012000400005Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2012-08-10T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
title |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
spellingShingle |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders Santos,Jansen R.P. Burrowing nematode in vitro multiplication root lesion nematode |
title_short |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
title_full |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
title_fullStr |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
title_full_unstemmed |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
title_sort |
Comparison of two methods for in vitro multiplication of Radopholus similis and Pratylenchus brachyurus in carrot cylinders |
author |
Santos,Jansen R.P. |
author_facet |
Santos,Jansen R.P. Andrade,Ednalva P. Costa,Dilson C. Gonzaga,Vilmar Cares,Juvenil E. |
author_role |
author |
author2 |
Andrade,Ednalva P. Costa,Dilson C. Gonzaga,Vilmar Cares,Juvenil E. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Santos,Jansen R.P. Andrade,Ednalva P. Costa,Dilson C. Gonzaga,Vilmar Cares,Juvenil E. |
dc.subject.por.fl_str_mv |
Burrowing nematode in vitro multiplication root lesion nematode |
topic |
Burrowing nematode in vitro multiplication root lesion nematode |
description |
The use of in vitro mass multiplication of nematodes under axenic conditions allows intensification of studies on taxonomy, biology, epidemiology and control of these pathogens. In the present study, two methods were compared for in vitro multiplication of two species of plant-parasitic nematodes, Radopholus similis and Pratylenchus brachyurus in 20, 40 and 60 day-periods. Both techniques involved the use of carrot cylinders placed in jars with lids, with water-agar in the bottom of the flasks (WA+) or without (WA-). In WA- the nematodes were treated with a 1% solution of ampicillin whilst for WA+ the axenization was carried out with a solution of mercuric chloride (0.01%), and streptomycin sulfate 0.02%. The WA+ condition was the most favorable for multiplication of both species of nematodes, resulting in a population increase of about 280 times for R. similis and 226 times for P. brachyurus in relation to the founding population of 25 individuals. For WA- a population increase of only five times after 60 days for R. similis and a duplication of the population after 60 days for P. brachyurus was obtained. Overall, the largest amount of nematodes occurred at 60 days after inoculation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762012000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.37 n.4 2012 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824585865330688 |