Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness

Detalhes bibliográficos
Autor(a) principal: Loureiro,Andreia
Data de Publicação: 2011
Outros Autores: Guerra-Guimarães,Leonor, Lidon,Fernando C., Bertrand,Benoit, Silva,Maria C., Várzea,Vítor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000500003
Resumo: The hemibiotrophic fungus Colletotrichum kahawae is the causal agent of Coffee Berry Disease - CBD, an economically devastating disease restricted to Arabica coffee production in Africa. Understanding pathogen variability is crucial for the implementation of disease control measures. In this study, six isoenzymatic systems (esterase, acid and alkaline phosphatase, malate dehydrogenase, peroxidase and superoxide dismutase) were used to assess the genetic variation among 12 C. kahawae isolates (from different geographic origins and with different levels of aggressiveness towards coffee) and one Colletotrichum gloeosporioides isolate (not pathogenic to green berries). Cluster analysis of the banding profiles obtained for the six enzymes enabled the differentiation of the two species and revealed the existence of intraspecific variability among C. kahawae isolates. Alkaline phosphatase was the most discriminative enzyme, allowing also the discrimination between the most and least aggressive isolates of C. kahawae.
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spelling Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressivenessCoffee berry diseaseisoelectric focusingisoenzymesvariabilityThe hemibiotrophic fungus Colletotrichum kahawae is the causal agent of Coffee Berry Disease - CBD, an economically devastating disease restricted to Arabica coffee production in Africa. Understanding pathogen variability is crucial for the implementation of disease control measures. In this study, six isoenzymatic systems (esterase, acid and alkaline phosphatase, malate dehydrogenase, peroxidase and superoxide dismutase) were used to assess the genetic variation among 12 C. kahawae isolates (from different geographic origins and with different levels of aggressiveness towards coffee) and one Colletotrichum gloeosporioides isolate (not pathogenic to green berries). Cluster analysis of the banding profiles obtained for the six enzymes enabled the differentiation of the two species and revealed the existence of intraspecific variability among C. kahawae isolates. Alkaline phosphatase was the most discriminative enzyme, allowing also the discrimination between the most and least aggressive isolates of C. kahawae.Sociedade Brasileira de Fitopatologia2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000500003Tropical Plant Pathology v.36 n.5 2011reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762011000500003info:eu-repo/semantics/openAccessLoureiro,AndreiaGuerra-Guimarães,LeonorLidon,Fernando C.Bertrand,BenoitSilva,Maria C.Várzea,Vítoreng2012-01-31T00:00:00Zoai:scielo:S1982-56762011000500003Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2012-01-31T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
title Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
spellingShingle Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
Loureiro,Andreia
Coffee berry disease
isoelectric focusing
isoenzymes
variability
title_short Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
title_full Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
title_fullStr Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
title_full_unstemmed Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
title_sort Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness
author Loureiro,Andreia
author_facet Loureiro,Andreia
Guerra-Guimarães,Leonor
Lidon,Fernando C.
Bertrand,Benoit
Silva,Maria C.
Várzea,Vítor
author_role author
author2 Guerra-Guimarães,Leonor
Lidon,Fernando C.
Bertrand,Benoit
Silva,Maria C.
Várzea,Vítor
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Loureiro,Andreia
Guerra-Guimarães,Leonor
Lidon,Fernando C.
Bertrand,Benoit
Silva,Maria C.
Várzea,Vítor
dc.subject.por.fl_str_mv Coffee berry disease
isoelectric focusing
isoenzymes
variability
topic Coffee berry disease
isoelectric focusing
isoenzymes
variability
description The hemibiotrophic fungus Colletotrichum kahawae is the causal agent of Coffee Berry Disease - CBD, an economically devastating disease restricted to Arabica coffee production in Africa. Understanding pathogen variability is crucial for the implementation of disease control measures. In this study, six isoenzymatic systems (esterase, acid and alkaline phosphatase, malate dehydrogenase, peroxidase and superoxide dismutase) were used to assess the genetic variation among 12 C. kahawae isolates (from different geographic origins and with different levels of aggressiveness towards coffee) and one Colletotrichum gloeosporioides isolate (not pathogenic to green berries). Cluster analysis of the banding profiles obtained for the six enzymes enabled the differentiation of the two species and revealed the existence of intraspecific variability among C. kahawae isolates. Alkaline phosphatase was the most discriminative enzyme, allowing also the discrimination between the most and least aggressive isolates of C. kahawae.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000500003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000500003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762011000500003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.36 n.5 2011
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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