Stress responses of tomato protoplasts to copper and paraquat
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200003 |
Resumo: | Plants are often exposed to external biotic or abiotic agents that can damage cells. Biotic agents include molecules produced by pathogens, and abiotic agents include ultraviolet radiation, heavy metals and xenobiotic agents such as herbicides. Because of the importance of abiotic stresses and the limited knowledge of the defense responses of plants to stresses, a study was conducted using flow cytometry to evaluate endocellular events in protoplasts of two tomato species, Lycopersicon hirsutum and Lycopersicon esculentum, following exposure to 10 mM CuCl2 and 1% paraquat. During the first 30 min of exposure to 10 mM CuCl2, mean fluorescence intensity values in both species decreased by more than 50% compared to the untreated control. During the first 30 min of paraquat treatment, the production of reactive oxygen species, evaluated as the incidence of protoplasts in which the superoxide anion was present, was 32.9 % and 25.4%, respectively, for L. hirsutum and L. esculentum. Hyperpolarization of the mitochondria for both tomatoes species was observed during the first 2 h. The absence of early membrane damage and the markedly detrimental effects of the treatments on the mitochondria suggest that additional mechanisms which lead to cell death may be involved in these processes, and that these mechanisms may be associated with apoptosis. |
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Stress responses of tomato protoplasts to copper and paraquatLycopersiconabiotic elicitationapoptosisprogrammed cell deathPlants are often exposed to external biotic or abiotic agents that can damage cells. Biotic agents include molecules produced by pathogens, and abiotic agents include ultraviolet radiation, heavy metals and xenobiotic agents such as herbicides. Because of the importance of abiotic stresses and the limited knowledge of the defense responses of plants to stresses, a study was conducted using flow cytometry to evaluate endocellular events in protoplasts of two tomato species, Lycopersicon hirsutum and Lycopersicon esculentum, following exposure to 10 mM CuCl2 and 1% paraquat. During the first 30 min of exposure to 10 mM CuCl2, mean fluorescence intensity values in both species decreased by more than 50% compared to the untreated control. During the first 30 min of paraquat treatment, the production of reactive oxygen species, evaluated as the incidence of protoplasts in which the superoxide anion was present, was 32.9 % and 25.4%, respectively, for L. hirsutum and L. esculentum. Hyperpolarization of the mitochondria for both tomatoes species was observed during the first 2 h. The absence of early membrane damage and the markedly detrimental effects of the treatments on the mitochondria suggest that additional mechanisms which lead to cell death may be involved in these processes, and that these mechanisms may be associated with apoptosis.Sociedade Brasileira de Fitopatologia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200003Tropical Plant Pathology v.36 n.2 2011reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762011000200003info:eu-repo/semantics/openAccessRueda,AntoniRojas,MauricioLobo,MarioUrrea,AuraRestrepo,CatalinaBotero,CatalinaPelaez,Carloseng2011-06-17T00:00:00Zoai:scielo:S1982-56762011000200003Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-06-17T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Stress responses of tomato protoplasts to copper and paraquat |
title |
Stress responses of tomato protoplasts to copper and paraquat |
spellingShingle |
Stress responses of tomato protoplasts to copper and paraquat Rueda,Antoni Lycopersicon abiotic elicitation apoptosis programmed cell death |
title_short |
Stress responses of tomato protoplasts to copper and paraquat |
title_full |
Stress responses of tomato protoplasts to copper and paraquat |
title_fullStr |
Stress responses of tomato protoplasts to copper and paraquat |
title_full_unstemmed |
Stress responses of tomato protoplasts to copper and paraquat |
title_sort |
Stress responses of tomato protoplasts to copper and paraquat |
author |
Rueda,Antoni |
author_facet |
Rueda,Antoni Rojas,Mauricio Lobo,Mario Urrea,Aura Restrepo,Catalina Botero,Catalina Pelaez,Carlos |
author_role |
author |
author2 |
Rojas,Mauricio Lobo,Mario Urrea,Aura Restrepo,Catalina Botero,Catalina Pelaez,Carlos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rueda,Antoni Rojas,Mauricio Lobo,Mario Urrea,Aura Restrepo,Catalina Botero,Catalina Pelaez,Carlos |
dc.subject.por.fl_str_mv |
Lycopersicon abiotic elicitation apoptosis programmed cell death |
topic |
Lycopersicon abiotic elicitation apoptosis programmed cell death |
description |
Plants are often exposed to external biotic or abiotic agents that can damage cells. Biotic agents include molecules produced by pathogens, and abiotic agents include ultraviolet radiation, heavy metals and xenobiotic agents such as herbicides. Because of the importance of abiotic stresses and the limited knowledge of the defense responses of plants to stresses, a study was conducted using flow cytometry to evaluate endocellular events in protoplasts of two tomato species, Lycopersicon hirsutum and Lycopersicon esculentum, following exposure to 10 mM CuCl2 and 1% paraquat. During the first 30 min of exposure to 10 mM CuCl2, mean fluorescence intensity values in both species decreased by more than 50% compared to the untreated control. During the first 30 min of paraquat treatment, the production of reactive oxygen species, evaluated as the incidence of protoplasts in which the superoxide anion was present, was 32.9 % and 25.4%, respectively, for L. hirsutum and L. esculentum. Hyperpolarization of the mitochondria for both tomatoes species was observed during the first 2 h. The absence of early membrane damage and the markedly detrimental effects of the treatments on the mitochondria suggest that additional mechanisms which lead to cell death may be involved in these processes, and that these mechanisms may be associated with apoptosis. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762011000200003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.36 n.2 2011 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824585415491584 |