Effect of neem seed extracts on the development of the Soybean Cysts Nematode
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762008000300001 |
Resumo: | The effect produced by neem seed extracts and a neem seed concentrate (NSC) on the mortality and development of Heterodera glycines was evaluated. Second-stage juvenile (J2) mortality produced by the extracts and NSC was determined. Soybean seedlings treated with each of the extracts (aqueous, methanolic and hexane) were inoculated with J2 and the number of females and eggs per root system determined after 30 days. Both the hexane extracts were ineffective as no statistical difference (P>0.05) in J2 mortality was observed between them and the control. No statistical difference (P>0.05) in J2 mortality (>98%) was obtained between the water extracts and methanol extract at 1000 mg L-1 treatments. The J2 percentage of mortality exponentially increased with the NSC dosages (R²=0.94). LD50 was 42.6 mg L-1 for NSC on H. glycines. In the greenhouse experiment, the highest reduction in the number of females was 84%, obtained with the aqueous (41.6 mg L-1) and methanolic extracts (1000 mg L-1). In the same way, the highest reduction in the number of eggs, of about 90%, was obtained with the same extracts. Thus, using aqueous extracts is more suitable than the remaining extracts in field conditions. The activities of the methanol and NSC extracts were attributed to the seven tetranortritepenoids (azadirachtin H, azadirachtin A, azadirachtin B, desacetylnimbin, desacetylsalannin, nimbin and salannin) identified by reverse phase high performance liquid chromatography. |
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Effect of neem seed extracts on the development of the Soybean Cysts NematodeAzadirachta indicaHeterodera glycinestetranortriterpenoidscontrolThe effect produced by neem seed extracts and a neem seed concentrate (NSC) on the mortality and development of Heterodera glycines was evaluated. Second-stage juvenile (J2) mortality produced by the extracts and NSC was determined. Soybean seedlings treated with each of the extracts (aqueous, methanolic and hexane) were inoculated with J2 and the number of females and eggs per root system determined after 30 days. Both the hexane extracts were ineffective as no statistical difference (P>0.05) in J2 mortality was observed between them and the control. No statistical difference (P>0.05) in J2 mortality (>98%) was obtained between the water extracts and methanol extract at 1000 mg L-1 treatments. The J2 percentage of mortality exponentially increased with the NSC dosages (R²=0.94). LD50 was 42.6 mg L-1 for NSC on H. glycines. In the greenhouse experiment, the highest reduction in the number of females was 84%, obtained with the aqueous (41.6 mg L-1) and methanolic extracts (1000 mg L-1). In the same way, the highest reduction in the number of eggs, of about 90%, was obtained with the same extracts. Thus, using aqueous extracts is more suitable than the remaining extracts in field conditions. The activities of the methanol and NSC extracts were attributed to the seven tetranortritepenoids (azadirachtin H, azadirachtin A, azadirachtin B, desacetylnimbin, desacetylsalannin, nimbin and salannin) identified by reverse phase high performance liquid chromatography.Sociedade Brasileira de Fitopatologia2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762008000300001Tropical Plant Pathology v.33 n.3 2008reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762008000300001info:eu-repo/semantics/openAccessSilva,Júlio C.T.Oliveira,Rosângela D.L.Jham,Gulab N.Aguiar,Naylor D.C.eng2008-07-23T00:00:00Zoai:scielo:S1982-56762008000300001Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2008-07-23T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
title |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
spellingShingle |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode Silva,Júlio C.T. Azadirachta indica Heterodera glycines tetranortriterpenoids control |
title_short |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
title_full |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
title_fullStr |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
title_full_unstemmed |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
title_sort |
Effect of neem seed extracts on the development of the Soybean Cysts Nematode |
author |
Silva,Júlio C.T. |
author_facet |
Silva,Júlio C.T. Oliveira,Rosângela D.L. Jham,Gulab N. Aguiar,Naylor D.C. |
author_role |
author |
author2 |
Oliveira,Rosângela D.L. Jham,Gulab N. Aguiar,Naylor D.C. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva,Júlio C.T. Oliveira,Rosângela D.L. Jham,Gulab N. Aguiar,Naylor D.C. |
dc.subject.por.fl_str_mv |
Azadirachta indica Heterodera glycines tetranortriterpenoids control |
topic |
Azadirachta indica Heterodera glycines tetranortriterpenoids control |
description |
The effect produced by neem seed extracts and a neem seed concentrate (NSC) on the mortality and development of Heterodera glycines was evaluated. Second-stage juvenile (J2) mortality produced by the extracts and NSC was determined. Soybean seedlings treated with each of the extracts (aqueous, methanolic and hexane) were inoculated with J2 and the number of females and eggs per root system determined after 30 days. Both the hexane extracts were ineffective as no statistical difference (P>0.05) in J2 mortality was observed between them and the control. No statistical difference (P>0.05) in J2 mortality (>98%) was obtained between the water extracts and methanol extract at 1000 mg L-1 treatments. The J2 percentage of mortality exponentially increased with the NSC dosages (R²=0.94). LD50 was 42.6 mg L-1 for NSC on H. glycines. In the greenhouse experiment, the highest reduction in the number of females was 84%, obtained with the aqueous (41.6 mg L-1) and methanolic extracts (1000 mg L-1). In the same way, the highest reduction in the number of eggs, of about 90%, was obtained with the same extracts. Thus, using aqueous extracts is more suitable than the remaining extracts in field conditions. The activities of the methanol and NSC extracts were attributed to the seven tetranortritepenoids (azadirachtin H, azadirachtin A, azadirachtin B, desacetylnimbin, desacetylsalannin, nimbin and salannin) identified by reverse phase high performance liquid chromatography. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762008000300001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762008000300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762008000300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.33 n.3 2008 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824584960409600 |