Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves

Detalhes bibliográficos
Autor(a) principal: Garrett,Rafael
Data de Publicação: 2012
Outros Autores: Romanos,Maria Teresa V., Borges,Ricardo M., Santos,Marcelo G., Rocha,Leandro, Silva,Antonio Jorge R. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200009
Resumo: This study describes the isolation of a flavonoid fraction from leaves of Ocotea notata (Nees & Mart.) Mez, Lauraceae, the identification of six major compounds (an A-type proanthocyanidin trimer [3], isoquercitrin [4], reynoutrin [5], miquelianin [6], quercitrin [7], afzelin [8]) and four minor compounds (catechin [1], epicatechin [2], quercetin [9], kaempferol [10]) present in the fraction and its activity against the Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The 50% effective concentrations values (EC50) calculated from the dose-response curve and the selectivity indices (SI) against the virus were: EC50 35.8 µg/mL and SI 5.5 to HSV-1 and EC50 23.5 µg/mL and SI 8.5 to HSV-2. The flavonoid fraction was more active against HSV-2 than HSV-1. The mechanisms of antiviral action of the flavonoid fraction against the virus were also evaluated. The percentage inhibition (PI) obtained for HSV-2 was higher than 90% in the following assays: virucidal, pre-treatment of cells, treatment of cells after viral adsorption and treatment of cells after viral penetration. For HSV-1, the flavonoid fraction had no effect in pre-treatment of cells and showed 60% of inhibition in virucidal assay.
id SBFGNOSIA-1_0ba4232aa42d489460d2b6821cf65ef7
oai_identifier_str oai:scielo:S0102-695X2012000200009
network_acronym_str SBFGNOSIA-1
network_name_str Revista Brasileira de Farmacognosia (Online)
repository_id_str
spelling Antiherpetic activity of a flavonoid fraction from Ocotea notata leavesantiherpesflavonoidsOcotea notataHSV-1HSV-2This study describes the isolation of a flavonoid fraction from leaves of Ocotea notata (Nees & Mart.) Mez, Lauraceae, the identification of six major compounds (an A-type proanthocyanidin trimer [3], isoquercitrin [4], reynoutrin [5], miquelianin [6], quercitrin [7], afzelin [8]) and four minor compounds (catechin [1], epicatechin [2], quercetin [9], kaempferol [10]) present in the fraction and its activity against the Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The 50% effective concentrations values (EC50) calculated from the dose-response curve and the selectivity indices (SI) against the virus were: EC50 35.8 µg/mL and SI 5.5 to HSV-1 and EC50 23.5 µg/mL and SI 8.5 to HSV-2. The flavonoid fraction was more active against HSV-2 than HSV-1. The mechanisms of antiviral action of the flavonoid fraction against the virus were also evaluated. The percentage inhibition (PI) obtained for HSV-2 was higher than 90% in the following assays: virucidal, pre-treatment of cells, treatment of cells after viral adsorption and treatment of cells after viral penetration. For HSV-1, the flavonoid fraction had no effect in pre-treatment of cells and showed 60% of inhibition in virucidal assay.Sociedade Brasileira de Farmacognosia2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200009Revista Brasileira de Farmacognosia v.22 n.2 2012reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2012005000003info:eu-repo/semantics/openAccessGarrett,RafaelRomanos,Maria Teresa V.Borges,Ricardo M.Santos,Marcelo G.Rocha,LeandroSilva,Antonio Jorge R. daeng2012-05-08T00:00:00Zoai:scielo:S0102-695X2012000200009Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2012-05-08T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
title Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
spellingShingle Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
Garrett,Rafael
antiherpes
flavonoids
Ocotea notata
HSV-1
HSV-2
title_short Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
title_full Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
title_fullStr Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
title_full_unstemmed Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
title_sort Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves
author Garrett,Rafael
author_facet Garrett,Rafael
Romanos,Maria Teresa V.
Borges,Ricardo M.
Santos,Marcelo G.
Rocha,Leandro
Silva,Antonio Jorge R. da
author_role author
author2 Romanos,Maria Teresa V.
Borges,Ricardo M.
Santos,Marcelo G.
Rocha,Leandro
Silva,Antonio Jorge R. da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Garrett,Rafael
Romanos,Maria Teresa V.
Borges,Ricardo M.
Santos,Marcelo G.
Rocha,Leandro
Silva,Antonio Jorge R. da
dc.subject.por.fl_str_mv antiherpes
flavonoids
Ocotea notata
HSV-1
HSV-2
topic antiherpes
flavonoids
Ocotea notata
HSV-1
HSV-2
description This study describes the isolation of a flavonoid fraction from leaves of Ocotea notata (Nees & Mart.) Mez, Lauraceae, the identification of six major compounds (an A-type proanthocyanidin trimer [3], isoquercitrin [4], reynoutrin [5], miquelianin [6], quercitrin [7], afzelin [8]) and four minor compounds (catechin [1], epicatechin [2], quercetin [9], kaempferol [10]) present in the fraction and its activity against the Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The 50% effective concentrations values (EC50) calculated from the dose-response curve and the selectivity indices (SI) against the virus were: EC50 35.8 µg/mL and SI 5.5 to HSV-1 and EC50 23.5 µg/mL and SI 8.5 to HSV-2. The flavonoid fraction was more active against HSV-2 than HSV-1. The mechanisms of antiviral action of the flavonoid fraction against the virus were also evaluated. The percentage inhibition (PI) obtained for HSV-2 was higher than 90% in the following assays: virucidal, pre-treatment of cells, treatment of cells after viral adsorption and treatment of cells after viral penetration. For HSV-1, the flavonoid fraction had no effect in pre-treatment of cells and showed 60% of inhibition in virucidal assay.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2012005000003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.22 n.2 2012
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
_version_ 1752122467409723392