Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000300325 |
Resumo: | ABSTRACT To evaluate the anti-Helicobacter pylori activity of the major polyphenol compounds of propolis and their cellular damage, both as single molecule or in combination. Honey bees propolis were fractionated by using CPC and preparative HPLC. Four major polyphenols (chrysin, pinocembrin, galangin and caffeic acid phenethyl ester) were identified by thin layer chromatography–mass spectroscopy and liquid chromatography–mass spectroscopy. These compounds inhibited both ATCC and clinical H. pylori strains, with caffeic acid phenethyl ester being the most active. The four compounds presented minimum inhibitory concentration in the range 256–1024 µg ml−1 and a fractional inhibitory concentration of 64–512 µg ml−1. In mixtures all compounds showed an indifference effect (FIC < 0.15) but chrysin + galangin which was synergistic (FIC = 2.0). Killing curves show a similar behavior as the antibiotic amoxycillin. On the other hand, analyses by transmission electron microscopy at sub inhibitory concentration show vesicle formation and cell lysis after exposition to both individual polyphenol compounds and in mixture. The major compounds of propolis show anti-H. pylori activity both as individual compounds and in mixture. When combined they present mainly indifference but exert a lytic activity upon H. pylori, suggesting a potential bactericidal activity of propolis. |
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Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitroPropolisCaffeic acid phenethyl esterGalanginIndifferenceMembrane vesiclesCellular lysisABSTRACT To evaluate the anti-Helicobacter pylori activity of the major polyphenol compounds of propolis and their cellular damage, both as single molecule or in combination. Honey bees propolis were fractionated by using CPC and preparative HPLC. Four major polyphenols (chrysin, pinocembrin, galangin and caffeic acid phenethyl ester) were identified by thin layer chromatography–mass spectroscopy and liquid chromatography–mass spectroscopy. These compounds inhibited both ATCC and clinical H. pylori strains, with caffeic acid phenethyl ester being the most active. The four compounds presented minimum inhibitory concentration in the range 256–1024 µg ml−1 and a fractional inhibitory concentration of 64–512 µg ml−1. In mixtures all compounds showed an indifference effect (FIC < 0.15) but chrysin + galangin which was synergistic (FIC = 2.0). Killing curves show a similar behavior as the antibiotic amoxycillin. On the other hand, analyses by transmission electron microscopy at sub inhibitory concentration show vesicle formation and cell lysis after exposition to both individual polyphenol compounds and in mixture. The major compounds of propolis show anti-H. pylori activity both as individual compounds and in mixture. When combined they present mainly indifference but exert a lytic activity upon H. pylori, suggesting a potential bactericidal activity of propolis.Sociedade Brasileira de Farmacognosia2019-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000300325Revista Brasileira de Farmacognosia v.29 n.3 2019reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2019.03.002info:eu-repo/semantics/openAccessRomero,MarioFreire,JoséPastene,EdgarGarcía,ApolinariaAranda,MarioGonzález,Carloseng2019-08-22T00:00:00Zoai:scielo:S0102-695X2019000300325Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2019-08-22T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
title |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
spellingShingle |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro Romero,Mario Propolis Caffeic acid phenethyl ester Galangin Indifference Membrane vesicles Cellular lysis |
title_short |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
title_full |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
title_fullStr |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
title_full_unstemmed |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
title_sort |
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro |
author |
Romero,Mario |
author_facet |
Romero,Mario Freire,José Pastene,Edgar García,Apolinaria Aranda,Mario González,Carlos |
author_role |
author |
author2 |
Freire,José Pastene,Edgar García,Apolinaria Aranda,Mario González,Carlos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Romero,Mario Freire,José Pastene,Edgar García,Apolinaria Aranda,Mario González,Carlos |
dc.subject.por.fl_str_mv |
Propolis Caffeic acid phenethyl ester Galangin Indifference Membrane vesicles Cellular lysis |
topic |
Propolis Caffeic acid phenethyl ester Galangin Indifference Membrane vesicles Cellular lysis |
description |
ABSTRACT To evaluate the anti-Helicobacter pylori activity of the major polyphenol compounds of propolis and their cellular damage, both as single molecule or in combination. Honey bees propolis were fractionated by using CPC and preparative HPLC. Four major polyphenols (chrysin, pinocembrin, galangin and caffeic acid phenethyl ester) were identified by thin layer chromatography–mass spectroscopy and liquid chromatography–mass spectroscopy. These compounds inhibited both ATCC and clinical H. pylori strains, with caffeic acid phenethyl ester being the most active. The four compounds presented minimum inhibitory concentration in the range 256–1024 µg ml−1 and a fractional inhibitory concentration of 64–512 µg ml−1. In mixtures all compounds showed an indifference effect (FIC < 0.15) but chrysin + galangin which was synergistic (FIC = 2.0). Killing curves show a similar behavior as the antibiotic amoxycillin. On the other hand, analyses by transmission electron microscopy at sub inhibitory concentration show vesicle formation and cell lysis after exposition to both individual polyphenol compounds and in mixture. The major compounds of propolis show anti-H. pylori activity both as individual compounds and in mixture. When combined they present mainly indifference but exert a lytic activity upon H. pylori, suggesting a potential bactericidal activity of propolis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000300325 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000300325 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2019.03.002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.29 n.3 2019 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122471149993984 |