Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

Detalhes bibliográficos
Autor(a) principal: Ducatti,Diogo R. B.
Data de Publicação: 2011
Outros Autores: Colodi,Franciely G., Gonçalves,Alan G., Duarte,M. Eugênia R., Noseda,Miguel D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015
Resumo: Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.
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spelling Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactansagaro-oligosaccharidescarra-oligosaccharidespartial acid hydrolysis3,6-anhydro-galactoseagarosekappa-carrageenanAgaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.Sociedade Brasileira de Farmacognosia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015Revista Brasileira de Farmacognosia v.21 n.2 2011reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2011005000080info:eu-repo/semantics/openAccessDucatti,Diogo R. B.Colodi,Franciely G.Gonçalves,Alan G.Duarte,M. Eugênia R.Noseda,Miguel D.eng2011-06-03T00:00:00Zoai:scielo:S0102-695X2011000200015Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2011-06-03T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
spellingShingle Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
Ducatti,Diogo R. B.
agaro-oligosaccharides
carra-oligosaccharides
partial acid hydrolysis
3,6-anhydro-galactose
agarose
kappa-carrageenan
title_short Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_full Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_fullStr Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_full_unstemmed Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
title_sort Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
author Ducatti,Diogo R. B.
author_facet Ducatti,Diogo R. B.
Colodi,Franciely G.
Gonçalves,Alan G.
Duarte,M. Eugênia R.
Noseda,Miguel D.
author_role author
author2 Colodi,Franciely G.
Gonçalves,Alan G.
Duarte,M. Eugênia R.
Noseda,Miguel D.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ducatti,Diogo R. B.
Colodi,Franciely G.
Gonçalves,Alan G.
Duarte,M. Eugênia R.
Noseda,Miguel D.
dc.subject.por.fl_str_mv agaro-oligosaccharides
carra-oligosaccharides
partial acid hydrolysis
3,6-anhydro-galactose
agarose
kappa-carrageenan
topic agaro-oligosaccharides
carra-oligosaccharides
partial acid hydrolysis
3,6-anhydro-galactose
agarose
kappa-carrageenan
description Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2011005000080
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.21 n.2 2011
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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