Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000600634 |
Resumo: | Abstract Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (L-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by L-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress. |
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Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cellsGorgonian coralMarine natural productsCytotoxicityQuinoneROSAbstract Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (L-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by L-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress.Sociedade Brasileira de Farmacognosia2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000600634Revista Brasileira de Farmacognosia v.25 n.6 2015reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2015.07.020info:eu-repo/semantics/openAccessAbreu,Paula A.Wilke,Diego V.Araujo,Ana J.Marinho-Filho,José Delano B.Ferreira,Elthon G.Ribeiro,Carlos Margo R.Pinheiro,Leandro S.Amorim,Juliana W.Valverde,Alessandra L.Epifanio,Rosângela A.Costa-Lotufo,Letícia V.Jimenez,Paula C.eng2015-12-22T00:00:00Zoai:scielo:S0102-695X2015000600634Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2015-12-22T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
title |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
spellingShingle |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells Abreu,Paula A. Gorgonian coral Marine natural products Cytotoxicity Quinone ROS |
title_short |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
title_full |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
title_fullStr |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
title_full_unstemmed |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
title_sort |
Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells |
author |
Abreu,Paula A. |
author_facet |
Abreu,Paula A. Wilke,Diego V. Araujo,Ana J. Marinho-Filho,José Delano B. Ferreira,Elthon G. Ribeiro,Carlos Margo R. Pinheiro,Leandro S. Amorim,Juliana W. Valverde,Alessandra L. Epifanio,Rosângela A. Costa-Lotufo,Letícia V. Jimenez,Paula C. |
author_role |
author |
author2 |
Wilke,Diego V. Araujo,Ana J. Marinho-Filho,José Delano B. Ferreira,Elthon G. Ribeiro,Carlos Margo R. Pinheiro,Leandro S. Amorim,Juliana W. Valverde,Alessandra L. Epifanio,Rosângela A. Costa-Lotufo,Letícia V. Jimenez,Paula C. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu,Paula A. Wilke,Diego V. Araujo,Ana J. Marinho-Filho,José Delano B. Ferreira,Elthon G. Ribeiro,Carlos Margo R. Pinheiro,Leandro S. Amorim,Juliana W. Valverde,Alessandra L. Epifanio,Rosângela A. Costa-Lotufo,Letícia V. Jimenez,Paula C. |
dc.subject.por.fl_str_mv |
Gorgonian coral Marine natural products Cytotoxicity Quinone ROS |
topic |
Gorgonian coral Marine natural products Cytotoxicity Quinone ROS |
description |
Abstract Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (L-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by L-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000600634 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000600634 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2015.07.020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.25 n.6 2015 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122469647384576 |