Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000500513 |
Resumo: | ABSTRACTChlorogenic and caffeic acids are bioactive phenolic compounds present in Cecropia glaziovii Snethl., Urticaceae, products that have been used as analytical markers. This paper reports a chemometric study aimed at improving chromatographic performance for quantification of these markers by RP-HPLC. The organic to aqueous content ratio, the acid content of the mobile phase, and the elution method were analyzed using a Response Surface Methodology IV-Optimal design. The resolution between peaks, retention time, tailing and retention factors, number of theoretical plates and peak widths were evaluated. The optimized conditions were mathematically determined as (A) trifluoroacetic acid 0.05% (v/v), (B) 12% (v/v) acetonitrile and (C) increasing gradient. The method was considered specific, fast, precise, reliable and linear in the ranges of 1.0–200.0 and 2.5–100.0 µg/ml for the chlorogenic and caffeic acids, respectively. The adequate conditions to separate and quantify both phenolic acids in C. glaziovii products were demonstrated. Satisfactory resolution was achieved when compared to a previously published chromatographic method which is unable to separate the chlorogenic acid and an interfering compound presented under certain extractive conditions, demonstrating the importance of systematic studies, specifically when analyzing complex plant matrices. |
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Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii productsRP-HPLC-UV methodResponse Surface MethodologyCecropia glazioviiVegetal extractChlorogenic acidCaffeic acidABSTRACTChlorogenic and caffeic acids are bioactive phenolic compounds present in Cecropia glaziovii Snethl., Urticaceae, products that have been used as analytical markers. This paper reports a chemometric study aimed at improving chromatographic performance for quantification of these markers by RP-HPLC. The organic to aqueous content ratio, the acid content of the mobile phase, and the elution method were analyzed using a Response Surface Methodology IV-Optimal design. The resolution between peaks, retention time, tailing and retention factors, number of theoretical plates and peak widths were evaluated. The optimized conditions were mathematically determined as (A) trifluoroacetic acid 0.05% (v/v), (B) 12% (v/v) acetonitrile and (C) increasing gradient. The method was considered specific, fast, precise, reliable and linear in the ranges of 1.0–200.0 and 2.5–100.0 µg/ml for the chlorogenic and caffeic acids, respectively. The adequate conditions to separate and quantify both phenolic acids in C. glaziovii products were demonstrated. Satisfactory resolution was achieved when compared to a previously published chromatographic method which is unable to separate the chlorogenic acid and an interfering compound presented under certain extractive conditions, demonstrating the importance of systematic studies, specifically when analyzing complex plant matrices.Sociedade Brasileira de Farmacognosia2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000500513Revista Brasileira de Farmacognosia v.25 n.5 2015reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2015.05.007info:eu-repo/semantics/openAccessBeringhs,André O.Dalmina,MileneCreczynski-Pasa,Tânia B.Sonaglio,Divaeng2015-11-11T00:00:00Zoai:scielo:S0102-695X2015000500513Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2015-11-11T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
title |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
spellingShingle |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products Beringhs,André O. RP-HPLC-UV method Response Surface Methodology Cecropia glaziovii Vegetal extract Chlorogenic acid Caffeic acid |
title_short |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
title_full |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
title_fullStr |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
title_full_unstemmed |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
title_sort |
Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products |
author |
Beringhs,André O. |
author_facet |
Beringhs,André O. Dalmina,Milene Creczynski-Pasa,Tânia B. Sonaglio,Diva |
author_role |
author |
author2 |
Dalmina,Milene Creczynski-Pasa,Tânia B. Sonaglio,Diva |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Beringhs,André O. Dalmina,Milene Creczynski-Pasa,Tânia B. Sonaglio,Diva |
dc.subject.por.fl_str_mv |
RP-HPLC-UV method Response Surface Methodology Cecropia glaziovii Vegetal extract Chlorogenic acid Caffeic acid |
topic |
RP-HPLC-UV method Response Surface Methodology Cecropia glaziovii Vegetal extract Chlorogenic acid Caffeic acid |
description |
ABSTRACTChlorogenic and caffeic acids are bioactive phenolic compounds present in Cecropia glaziovii Snethl., Urticaceae, products that have been used as analytical markers. This paper reports a chemometric study aimed at improving chromatographic performance for quantification of these markers by RP-HPLC. The organic to aqueous content ratio, the acid content of the mobile phase, and the elution method were analyzed using a Response Surface Methodology IV-Optimal design. The resolution between peaks, retention time, tailing and retention factors, number of theoretical plates and peak widths were evaluated. The optimized conditions were mathematically determined as (A) trifluoroacetic acid 0.05% (v/v), (B) 12% (v/v) acetonitrile and (C) increasing gradient. The method was considered specific, fast, precise, reliable and linear in the ranges of 1.0–200.0 and 2.5–100.0 µg/ml for the chlorogenic and caffeic acids, respectively. The adequate conditions to separate and quantify both phenolic acids in C. glaziovii products were demonstrated. Satisfactory resolution was achieved when compared to a previously published chromatographic method which is unable to separate the chlorogenic acid and an interfering compound presented under certain extractive conditions, demonstrating the importance of systematic studies, specifically when analyzing complex plant matrices. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000500513 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000500513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2015.05.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.25 n.5 2015 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122469624315904 |