Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100118 |
Resumo: | ABSTRACT Hypericum species, Hypericaceae, are recognized as a source of therapeutical agents. Purified fractions and isolated compounds have been shown antimicrobial activity. As the indiscriminate use of antifungals and the increase of infections caused by emerging species are leading to the search of new alternative treatments, the aim of this study was to continue the study with Hypericum carinatum Griseb. lipophilic fraction, rich in phloroglucinol derivatives, investigating the effect of its association with fluconazole against emerging yeasts (Candida krusei, C. famata, C. parapsilosis and Cryptococcus neoformans). The synergistic activity between H. carinatum lipophilic fraction and fluconazole was assessed by two methodologies for multiple dose–response analysis: checkerboard and isobologram. Regarding synergistic experiments, the effect of the association was higher than the effect of fluconazole alone against Candida krusei and C. famata isolates (MIC fluconazole decreased about eight and four folds, respectively), suggesting that, somehow, H. carinatum lipophilic fraction compounds are facilitating the action of this drug. On the other hand, when tested against Cryptococcus neoformans and C. parapsilosis, fluconazole showed better results than the association. Thus, against Candida krusei and C. famata, the lipophilic fraction of H. carinatum was able to reduce the MIC values of fluconazole and could be considered as a potential alternative to be used against emerging yeast species. |
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Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazoleCandidaCheckerboardFluconazoleHypericum carinatumIsobologramSynergistic activityABSTRACT Hypericum species, Hypericaceae, are recognized as a source of therapeutical agents. Purified fractions and isolated compounds have been shown antimicrobial activity. As the indiscriminate use of antifungals and the increase of infections caused by emerging species are leading to the search of new alternative treatments, the aim of this study was to continue the study with Hypericum carinatum Griseb. lipophilic fraction, rich in phloroglucinol derivatives, investigating the effect of its association with fluconazole against emerging yeasts (Candida krusei, C. famata, C. parapsilosis and Cryptococcus neoformans). The synergistic activity between H. carinatum lipophilic fraction and fluconazole was assessed by two methodologies for multiple dose–response analysis: checkerboard and isobologram. Regarding synergistic experiments, the effect of the association was higher than the effect of fluconazole alone against Candida krusei and C. famata isolates (MIC fluconazole decreased about eight and four folds, respectively), suggesting that, somehow, H. carinatum lipophilic fraction compounds are facilitating the action of this drug. On the other hand, when tested against Cryptococcus neoformans and C. parapsilosis, fluconazole showed better results than the association. Thus, against Candida krusei and C. famata, the lipophilic fraction of H. carinatum was able to reduce the MIC values of fluconazole and could be considered as a potential alternative to be used against emerging yeast species.Sociedade Brasileira de Farmacognosia2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100118Revista Brasileira de Farmacognosia v.27 n.1 2017reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2016.08.001info:eu-repo/semantics/openAccessMeirelles,Gabriela C.Pippi,BrunaHatwig,CamilaBarros,Francisco M.C. deOliveira,Luis F.S. dePoser,Gilsane L. vonFuentefria,Alexandre M.eng2017-01-10T00:00:00Zoai:scielo:S0102-695X2017000100118Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2017-01-10T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
title |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
spellingShingle |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole Meirelles,Gabriela C. Candida Checkerboard Fluconazole Hypericum carinatum Isobologram Synergistic activity |
title_short |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
title_full |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
title_fullStr |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
title_full_unstemmed |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
title_sort |
Synergistic antifungal activity of the lipophilic fraction of Hypericum carinatum and fluconazole |
author |
Meirelles,Gabriela C. |
author_facet |
Meirelles,Gabriela C. Pippi,Bruna Hatwig,Camila Barros,Francisco M.C. de Oliveira,Luis F.S. de Poser,Gilsane L. von Fuentefria,Alexandre M. |
author_role |
author |
author2 |
Pippi,Bruna Hatwig,Camila Barros,Francisco M.C. de Oliveira,Luis F.S. de Poser,Gilsane L. von Fuentefria,Alexandre M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Meirelles,Gabriela C. Pippi,Bruna Hatwig,Camila Barros,Francisco M.C. de Oliveira,Luis F.S. de Poser,Gilsane L. von Fuentefria,Alexandre M. |
dc.subject.por.fl_str_mv |
Candida Checkerboard Fluconazole Hypericum carinatum Isobologram Synergistic activity |
topic |
Candida Checkerboard Fluconazole Hypericum carinatum Isobologram Synergistic activity |
description |
ABSTRACT Hypericum species, Hypericaceae, are recognized as a source of therapeutical agents. Purified fractions and isolated compounds have been shown antimicrobial activity. As the indiscriminate use of antifungals and the increase of infections caused by emerging species are leading to the search of new alternative treatments, the aim of this study was to continue the study with Hypericum carinatum Griseb. lipophilic fraction, rich in phloroglucinol derivatives, investigating the effect of its association with fluconazole against emerging yeasts (Candida krusei, C. famata, C. parapsilosis and Cryptococcus neoformans). The synergistic activity between H. carinatum lipophilic fraction and fluconazole was assessed by two methodologies for multiple dose–response analysis: checkerboard and isobologram. Regarding synergistic experiments, the effect of the association was higher than the effect of fluconazole alone against Candida krusei and C. famata isolates (MIC fluconazole decreased about eight and four folds, respectively), suggesting that, somehow, H. carinatum lipophilic fraction compounds are facilitating the action of this drug. On the other hand, when tested against Cryptococcus neoformans and C. parapsilosis, fluconazole showed better results than the association. Thus, against Candida krusei and C. famata, the lipophilic fraction of H. carinatum was able to reduce the MIC values of fluconazole and could be considered as a potential alternative to be used against emerging yeast species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100118 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100118 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2016.08.001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.27 n.1 2017 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122470168526848 |