Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines

Detalhes bibliográficos
Autor(a) principal: Thomas,Anvy Susan
Data de Publicação: 2017
Outros Autores: Saravanakumar,Rupachandra, Gupta,Pratiksha V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100099
Resumo: ABSTRACT Biologically active proteins isolated from plant species can be used in traditional medicine as prolific resources for new drugs Morinda pubescens Sm., Rubiaceae, is a promising medicinal plant which is widely used in folk medicine to treat fever due to primary complex, ulcer and glandular swellings. In this study, proteins were extracted from the leaves of M. pubescens, and precipitated with ammonium sulphate at various saturation concentrations ranging from 20 to 80%. The precipitated protein sample obtained with 80% saturation was further purified using ultrafiltration membrane (<10 kDa). SDS-PAGE analysis identified the presence of crude and ultrafiltered protein bands. FTIR spectrum of the ultrafiltered protein fractions depicted the presence of hydroxyl and carbonyl groups of proteins. The ultrafiltered proteins exhibited increased cytotoxic activity on A549 cells at the concentrations ranging from 15 to 100 µg/ml. About 98% cell viability was also observed in Vero cells treated with the maximum concentration of 100 µg/ml of ultrafiltered protein extract. DNA fragmentation was observed in A549 cells treated with 10 µg/ml of ultrafiltered proteins, indicating the onset of apoptosis.
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spelling Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell linesCytotoxic activityFTIR analysisMorinda pubescensMTT assayProtein extractionABSTRACT Biologically active proteins isolated from plant species can be used in traditional medicine as prolific resources for new drugs Morinda pubescens Sm., Rubiaceae, is a promising medicinal plant which is widely used in folk medicine to treat fever due to primary complex, ulcer and glandular swellings. In this study, proteins were extracted from the leaves of M. pubescens, and precipitated with ammonium sulphate at various saturation concentrations ranging from 20 to 80%. The precipitated protein sample obtained with 80% saturation was further purified using ultrafiltration membrane (<10 kDa). SDS-PAGE analysis identified the presence of crude and ultrafiltered protein bands. FTIR spectrum of the ultrafiltered protein fractions depicted the presence of hydroxyl and carbonyl groups of proteins. The ultrafiltered proteins exhibited increased cytotoxic activity on A549 cells at the concentrations ranging from 15 to 100 µg/ml. About 98% cell viability was also observed in Vero cells treated with the maximum concentration of 100 µg/ml of ultrafiltered protein extract. DNA fragmentation was observed in A549 cells treated with 10 µg/ml of ultrafiltered proteins, indicating the onset of apoptosis.Sociedade Brasileira de Farmacognosia2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100099Revista Brasileira de Farmacognosia v.27 n.1 2017reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2016.08.003info:eu-repo/semantics/openAccessThomas,Anvy SusanSaravanakumar,RupachandraGupta,Pratiksha V.eng2017-01-10T00:00:00Zoai:scielo:S0102-695X2017000100099Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2017-01-10T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
title Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
spellingShingle Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
Thomas,Anvy Susan
Cytotoxic activity
FTIR analysis
Morinda pubescens
MTT assay
Protein extraction
title_short Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
title_full Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
title_fullStr Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
title_full_unstemmed Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
title_sort Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
author Thomas,Anvy Susan
author_facet Thomas,Anvy Susan
Saravanakumar,Rupachandra
Gupta,Pratiksha V.
author_role author
author2 Saravanakumar,Rupachandra
Gupta,Pratiksha V.
author2_role author
author
dc.contributor.author.fl_str_mv Thomas,Anvy Susan
Saravanakumar,Rupachandra
Gupta,Pratiksha V.
dc.subject.por.fl_str_mv Cytotoxic activity
FTIR analysis
Morinda pubescens
MTT assay
Protein extraction
topic Cytotoxic activity
FTIR analysis
Morinda pubescens
MTT assay
Protein extraction
description ABSTRACT Biologically active proteins isolated from plant species can be used in traditional medicine as prolific resources for new drugs Morinda pubescens Sm., Rubiaceae, is a promising medicinal plant which is widely used in folk medicine to treat fever due to primary complex, ulcer and glandular swellings. In this study, proteins were extracted from the leaves of M. pubescens, and precipitated with ammonium sulphate at various saturation concentrations ranging from 20 to 80%. The precipitated protein sample obtained with 80% saturation was further purified using ultrafiltration membrane (<10 kDa). SDS-PAGE analysis identified the presence of crude and ultrafiltered protein bands. FTIR spectrum of the ultrafiltered protein fractions depicted the presence of hydroxyl and carbonyl groups of proteins. The ultrafiltered proteins exhibited increased cytotoxic activity on A549 cells at the concentrations ranging from 15 to 100 µg/ml. About 98% cell viability was also observed in Vero cells treated with the maximum concentration of 100 µg/ml of ultrafiltered protein extract. DNA fragmentation was observed in A549 cells treated with 10 µg/ml of ultrafiltered proteins, indicating the onset of apoptosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100099
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjp.2016.08.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.27 n.1 2017
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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