Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000100011 |
Resumo: | Tridax procumbens L., Asteraceae, has been extensively used in Ayurvedic system of medicine for various ailments. Previous studies on the extracts of T. procumbens revealed remarkable immunomodulatory activity of TPEIF (T. procumbens ethanol insoluble fraction) extract. The dried methanol extract of T. procumbens was dissolved in distilled water, and then fractioned by re-extracting with chloroform, ethyl acetate and n-butanol subsequently. Immunomodulatory activities of these fractions were determined in vivo. The amounts of total phenolic compounds were also determined. Ethyl acetate and n-butanol fractions showed the significant immunomodulary activity. However, the ethyl acetate fraction exhibited the highest total phenolic content. Therefore, ethyl acetate fraction was subjected to further separation by chromatographic methods. Two phytochemicals SA-3 and SA-4 were obtained by repeated purification in sufficient amount to screen them for the immunomodulatory activity by the in vivo models i.e. neutrophil adhesion and delayed type hypersensitivity. In addition, the n-butanol fraction was subjected to silica gel column chromatography (CC); SA-6 was isolated from it. Mice were treated with two doses of SA-3, SA-4 and SA-6 (2 and 4 mg/kg) for fifteen days. Immune responses to T-dependent antigen SRBCs were observed using parameters like DTH and Neutrophil adhesion. Overall, SA-4 and SA-6 showed dose relative immunostimulatory effect on in vivo immune functions in mice. From these results, it can be suggested that these compounds may be used as potential immunostimulators. The structures of isolated phytochemicals were determined by UV, IR, NMR, and MS spectroscopic methods. |
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Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbensimmunomodulatory activityisolationdelayed hypersensitivityneutrophil adhesionTridax procumbensTridax procumbens L., Asteraceae, has been extensively used in Ayurvedic system of medicine for various ailments. Previous studies on the extracts of T. procumbens revealed remarkable immunomodulatory activity of TPEIF (T. procumbens ethanol insoluble fraction) extract. The dried methanol extract of T. procumbens was dissolved in distilled water, and then fractioned by re-extracting with chloroform, ethyl acetate and n-butanol subsequently. Immunomodulatory activities of these fractions were determined in vivo. The amounts of total phenolic compounds were also determined. Ethyl acetate and n-butanol fractions showed the significant immunomodulary activity. However, the ethyl acetate fraction exhibited the highest total phenolic content. Therefore, ethyl acetate fraction was subjected to further separation by chromatographic methods. Two phytochemicals SA-3 and SA-4 were obtained by repeated purification in sufficient amount to screen them for the immunomodulatory activity by the in vivo models i.e. neutrophil adhesion and delayed type hypersensitivity. In addition, the n-butanol fraction was subjected to silica gel column chromatography (CC); SA-6 was isolated from it. Mice were treated with two doses of SA-3, SA-4 and SA-6 (2 and 4 mg/kg) for fifteen days. Immune responses to T-dependent antigen SRBCs were observed using parameters like DTH and Neutrophil adhesion. Overall, SA-4 and SA-6 showed dose relative immunostimulatory effect on in vivo immune functions in mice. From these results, it can be suggested that these compounds may be used as potential immunostimulators. The structures of isolated phytochemicals were determined by UV, IR, NMR, and MS spectroscopic methods.Sociedade Brasileira de Farmacognosia2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000100011Revista Brasileira de Farmacognosia v.21 n.1 2011reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2011005000011info:eu-repo/semantics/openAccessAgrawal,Surendra S.Talele,Gokul S.eng2011-03-28T00:00:00Zoai:scielo:S0102-695X2011000100011Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2011-03-28T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
title |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
spellingShingle |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens Agrawal,Surendra S. immunomodulatory activity isolation delayed hypersensitivity neutrophil adhesion Tridax procumbens |
title_short |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
title_full |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
title_fullStr |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
title_full_unstemmed |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
title_sort |
Bioactivity guided isolation and characterization of the phytoconstituents from the Tridax procumbens |
author |
Agrawal,Surendra S. |
author_facet |
Agrawal,Surendra S. Talele,Gokul S. |
author_role |
author |
author2 |
Talele,Gokul S. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Agrawal,Surendra S. Talele,Gokul S. |
dc.subject.por.fl_str_mv |
immunomodulatory activity isolation delayed hypersensitivity neutrophil adhesion Tridax procumbens |
topic |
immunomodulatory activity isolation delayed hypersensitivity neutrophil adhesion Tridax procumbens |
description |
Tridax procumbens L., Asteraceae, has been extensively used in Ayurvedic system of medicine for various ailments. Previous studies on the extracts of T. procumbens revealed remarkable immunomodulatory activity of TPEIF (T. procumbens ethanol insoluble fraction) extract. The dried methanol extract of T. procumbens was dissolved in distilled water, and then fractioned by re-extracting with chloroform, ethyl acetate and n-butanol subsequently. Immunomodulatory activities of these fractions were determined in vivo. The amounts of total phenolic compounds were also determined. Ethyl acetate and n-butanol fractions showed the significant immunomodulary activity. However, the ethyl acetate fraction exhibited the highest total phenolic content. Therefore, ethyl acetate fraction was subjected to further separation by chromatographic methods. Two phytochemicals SA-3 and SA-4 were obtained by repeated purification in sufficient amount to screen them for the immunomodulatory activity by the in vivo models i.e. neutrophil adhesion and delayed type hypersensitivity. In addition, the n-butanol fraction was subjected to silica gel column chromatography (CC); SA-6 was isolated from it. Mice were treated with two doses of SA-3, SA-4 and SA-6 (2 and 4 mg/kg) for fifteen days. Immune responses to T-dependent antigen SRBCs were observed using parameters like DTH and Neutrophil adhesion. Overall, SA-4 and SA-6 showed dose relative immunostimulatory effect on in vivo immune functions in mice. From these results, it can be suggested that these compounds may be used as potential immunostimulators. The structures of isolated phytochemicals were determined by UV, IR, NMR, and MS spectroscopic methods. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000100011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2011005000011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.21 n.1 2011 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
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1752122465614561280 |