Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000300219 |
Resumo: | AbstractThis study uses high performance liquid chromatography and capillary electrophoresis as analytical tools to evaluate flavonoids in hydrolyzed leaves extracts of Rubus erythrocladus Mart., Rosaceae, and Morus nigra L., Moraceae. For phytochemical analysis, the extracts were prepared by acid hydrolysis and ultrasonic bath and analyzed by high performance liquid chromatography using an ultraviolet detector and by capillary electrophoresis equipped with a diode-array detector. Quercetin and kaempferol were identified in these extracts. The analytical methods developed were validated and applied. Quercetin and kaempferol were quantified in R. erythrocladus, with 848.43 ± 66.68 μg g-1 and 304.35 ± 17.29 μg g-1, respectively, by HPLC-UV and quercetin, 836.37 ± 149.43 μg g-1, by CE-DAD. In M. nigra the quantifications of quercetin and kaempferol were 2323.90 ± 145.35 μg g-1 and 1446.36 ± 59.00 μg g-1, respectively, by HPLC-UV and, 2552.82 ± 275.30 μg g-1 and 1188.67 ± 99.21 μg g-1, respectively, by CE-DAD. The extracts were also analyzed by ultra-performance liquid chromatography coupled with a diode-array detector and mass spectrometer (MS), UPLC-DAD/MS. |
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Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresisHPLC-UVUPLC-DAD/MSCE-DADRubus erythrocladusMorus nigraFlavonoidsAbstractThis study uses high performance liquid chromatography and capillary electrophoresis as analytical tools to evaluate flavonoids in hydrolyzed leaves extracts of Rubus erythrocladus Mart., Rosaceae, and Morus nigra L., Moraceae. For phytochemical analysis, the extracts were prepared by acid hydrolysis and ultrasonic bath and analyzed by high performance liquid chromatography using an ultraviolet detector and by capillary electrophoresis equipped with a diode-array detector. Quercetin and kaempferol were identified in these extracts. The analytical methods developed were validated and applied. Quercetin and kaempferol were quantified in R. erythrocladus, with 848.43 ± 66.68 μg g-1 and 304.35 ± 17.29 μg g-1, respectively, by HPLC-UV and quercetin, 836.37 ± 149.43 μg g-1, by CE-DAD. In M. nigra the quantifications of quercetin and kaempferol were 2323.90 ± 145.35 μg g-1 and 1446.36 ± 59.00 μg g-1, respectively, by HPLC-UV and, 2552.82 ± 275.30 μg g-1 and 1188.67 ± 99.21 μg g-1, respectively, by CE-DAD. The extracts were also analyzed by ultra-performance liquid chromatography coupled with a diode-array detector and mass spectrometer (MS), UPLC-DAD/MS.Sociedade Brasileira de Farmacognosia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000300219Revista Brasileira de Farmacognosia v.25 n.3 2015reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2015.04.003info:eu-repo/semantics/openAccessTallini,Luciana R.Pedrazza,Graziele P.R.Bordignon,Sérgio A. de L.Costa,Ana C.O.Steppe,MartinFuentefria,AlexandreZuanazzi,José A.S.eng2015-08-27T00:00:00Zoai:scielo:S0102-695X2015000300219Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2015-08-27T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
title |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
spellingShingle |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis Tallini,Luciana R. HPLC-UV UPLC-DAD/MS CE-DAD Rubus erythrocladus Morus nigra Flavonoids |
title_short |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
title_full |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
title_fullStr |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
title_full_unstemmed |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
title_sort |
Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis |
author |
Tallini,Luciana R. |
author_facet |
Tallini,Luciana R. Pedrazza,Graziele P.R. Bordignon,Sérgio A. de L. Costa,Ana C.O. Steppe,Martin Fuentefria,Alexandre Zuanazzi,José A.S. |
author_role |
author |
author2 |
Pedrazza,Graziele P.R. Bordignon,Sérgio A. de L. Costa,Ana C.O. Steppe,Martin Fuentefria,Alexandre Zuanazzi,José A.S. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Tallini,Luciana R. Pedrazza,Graziele P.R. Bordignon,Sérgio A. de L. Costa,Ana C.O. Steppe,Martin Fuentefria,Alexandre Zuanazzi,José A.S. |
dc.subject.por.fl_str_mv |
HPLC-UV UPLC-DAD/MS CE-DAD Rubus erythrocladus Morus nigra Flavonoids |
topic |
HPLC-UV UPLC-DAD/MS CE-DAD Rubus erythrocladus Morus nigra Flavonoids |
description |
AbstractThis study uses high performance liquid chromatography and capillary electrophoresis as analytical tools to evaluate flavonoids in hydrolyzed leaves extracts of Rubus erythrocladus Mart., Rosaceae, and Morus nigra L., Moraceae. For phytochemical analysis, the extracts were prepared by acid hydrolysis and ultrasonic bath and analyzed by high performance liquid chromatography using an ultraviolet detector and by capillary electrophoresis equipped with a diode-array detector. Quercetin and kaempferol were identified in these extracts. The analytical methods developed were validated and applied. Quercetin and kaempferol were quantified in R. erythrocladus, with 848.43 ± 66.68 μg g-1 and 304.35 ± 17.29 μg g-1, respectively, by HPLC-UV and quercetin, 836.37 ± 149.43 μg g-1, by CE-DAD. In M. nigra the quantifications of quercetin and kaempferol were 2323.90 ± 145.35 μg g-1 and 1446.36 ± 59.00 μg g-1, respectively, by HPLC-UV and, 2552.82 ± 275.30 μg g-1 and 1188.67 ± 99.21 μg g-1, respectively, by CE-DAD. The extracts were also analyzed by ultra-performance liquid chromatography coupled with a diode-array detector and mass spectrometer (MS), UPLC-DAD/MS. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000300219 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2015000300219 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2015.04.003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.25 n.3 2015 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122469262557184 |