A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2007000300005 |
Resumo: | Chrysobalanus icaco (C. icaco) leaves are used in folk medicine (known as Abajeru in Brazil) to control the glycaemia in diabetic patients. Stannous chloride (SnCl2) is a powerful reducing agent used for different purposes and presents cytotoxic and genotoxic effects. The aim of this work was to investigate the effect of an aqueous C. icaco extract on the plasmid DNA topology and on the effects of the stannous chloride on DNA plasmid. Plasmid pBSK was incubated with a C. icaco extract in the presence or absence of SnCl2 (200 mg/mL), after that, the agarose gel electrophoresis procedure was carried out. Plasmid incubated only SnCl2 was used as positive control and, as negative control, plasmid incubated with Tris buffer. The gels were stained with ethidium bromide, DNA bands were semiquantified by densitometry. The data showed that C. icaco extract alters the electrophoretic profile and decreases significantly (p < 0.05) the effect of SnCl2 on plasmid DNA. The results obtained in this work could indicate a dose-dependent protective action and a genotoxic effect of C. icaco extract on plasmid DNA. |
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A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmidsChrysobalanus icacoChrysobalanaceaeplasmid DNAstannous chloridegenotoxic effectantioxidantChrysobalanus icaco (C. icaco) leaves are used in folk medicine (known as Abajeru in Brazil) to control the glycaemia in diabetic patients. Stannous chloride (SnCl2) is a powerful reducing agent used for different purposes and presents cytotoxic and genotoxic effects. The aim of this work was to investigate the effect of an aqueous C. icaco extract on the plasmid DNA topology and on the effects of the stannous chloride on DNA plasmid. Plasmid pBSK was incubated with a C. icaco extract in the presence or absence of SnCl2 (200 mg/mL), after that, the agarose gel electrophoresis procedure was carried out. Plasmid incubated only SnCl2 was used as positive control and, as negative control, plasmid incubated with Tris buffer. The gels were stained with ethidium bromide, DNA bands were semiquantified by densitometry. The data showed that C. icaco extract alters the electrophoretic profile and decreases significantly (p < 0.05) the effect of SnCl2 on plasmid DNA. The results obtained in this work could indicate a dose-dependent protective action and a genotoxic effect of C. icaco extract on plasmid DNA.Sociedade Brasileira de Farmacognosia2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2007000300005Revista Brasileira de Farmacognosia v.17 n.3 2007reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2007000300005info:eu-repo/semantics/openAccessPresta,Giuseppe A.Fonseca,Adenilson S.Bernardo-Filho,Marioeng2007-10-11T00:00:00Zoai:scielo:S0102-695X2007000300005Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2007-10-11T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
title |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
spellingShingle |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids Presta,Giuseppe A. Chrysobalanus icaco Chrysobalanaceae plasmid DNA stannous chloride genotoxic effect antioxidant |
title_short |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
title_full |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
title_fullStr |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
title_full_unstemmed |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
title_sort |
A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids |
author |
Presta,Giuseppe A. |
author_facet |
Presta,Giuseppe A. Fonseca,Adenilson S. Bernardo-Filho,Mario |
author_role |
author |
author2 |
Fonseca,Adenilson S. Bernardo-Filho,Mario |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Presta,Giuseppe A. Fonseca,Adenilson S. Bernardo-Filho,Mario |
dc.subject.por.fl_str_mv |
Chrysobalanus icaco Chrysobalanaceae plasmid DNA stannous chloride genotoxic effect antioxidant |
topic |
Chrysobalanus icaco Chrysobalanaceae plasmid DNA stannous chloride genotoxic effect antioxidant |
description |
Chrysobalanus icaco (C. icaco) leaves are used in folk medicine (known as Abajeru in Brazil) to control the glycaemia in diabetic patients. Stannous chloride (SnCl2) is a powerful reducing agent used for different purposes and presents cytotoxic and genotoxic effects. The aim of this work was to investigate the effect of an aqueous C. icaco extract on the plasmid DNA topology and on the effects of the stannous chloride on DNA plasmid. Plasmid pBSK was incubated with a C. icaco extract in the presence or absence of SnCl2 (200 mg/mL), after that, the agarose gel electrophoresis procedure was carried out. Plasmid incubated only SnCl2 was used as positive control and, as negative control, plasmid incubated with Tris buffer. The gels were stained with ethidium bromide, DNA bands were semiquantified by densitometry. The data showed that C. icaco extract alters the electrophoretic profile and decreases significantly (p < 0.05) the effect of SnCl2 on plasmid DNA. The results obtained in this work could indicate a dose-dependent protective action and a genotoxic effect of C. icaco extract on plasmid DNA. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2007000300005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2007000300005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2007000300005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.17 n.3 2007 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122462566350848 |