Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures

Detalhes bibliográficos
Autor(a) principal: Mathur,Manas
Data de Publicação: 2012
Outros Autores: Kamal,Raka
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000500010
Resumo: Trigonelline (N-methylnicotinate) biosynthesized from nicotinate is one of the metabolically active pyridine alkaloid, widely distributed in plant kingdom. In the present study trigonelline has been isolated from various plant parts and callus cultures of Moringa oleifera Lam., Moringaceae, and was identified using TLC, GLC, GC-MS, which was comparable to that of the standard trigonelline. The trigonelline recovery was found to be maximum in the pods and minimum in flowers. In order to enhance the production of trigonelline in vitro grown cultures, different treatment doses of nicotinic acid (250, 500 and 750 mg L-1) were supplemented in the medium as precursor. Maximum increase (up to 1.10 fold) was observed in the treatment dose of 500 mg L-1 of nicotinic acid.
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spelling Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell culturescallus cultureGC-MSMoringa oleiferanicotinic acidTLCtrigonellineTrigonelline (N-methylnicotinate) biosynthesized from nicotinate is one of the metabolically active pyridine alkaloid, widely distributed in plant kingdom. In the present study trigonelline has been isolated from various plant parts and callus cultures of Moringa oleifera Lam., Moringaceae, and was identified using TLC, GLC, GC-MS, which was comparable to that of the standard trigonelline. The trigonelline recovery was found to be maximum in the pods and minimum in flowers. In order to enhance the production of trigonelline in vitro grown cultures, different treatment doses of nicotinic acid (250, 500 and 750 mg L-1) were supplemented in the medium as precursor. Maximum increase (up to 1.10 fold) was observed in the treatment dose of 500 mg L-1 of nicotinic acid.Sociedade Brasileira de Farmacognosia2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000500010Revista Brasileira de Farmacognosia v.22 n.5 2012reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2012005000041info:eu-repo/semantics/openAccessMathur,ManasKamal,Rakaeng2012-09-18T00:00:00Zoai:scielo:S0102-695X2012000500010Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2012-09-18T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
title Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
spellingShingle Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
Mathur,Manas
callus culture
GC-MS
Moringa oleifera
nicotinic acid
TLC
trigonelline
title_short Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
title_full Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
title_fullStr Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
title_full_unstemmed Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
title_sort Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures
author Mathur,Manas
author_facet Mathur,Manas
Kamal,Raka
author_role author
author2 Kamal,Raka
author2_role author
dc.contributor.author.fl_str_mv Mathur,Manas
Kamal,Raka
dc.subject.por.fl_str_mv callus culture
GC-MS
Moringa oleifera
nicotinic acid
TLC
trigonelline
topic callus culture
GC-MS
Moringa oleifera
nicotinic acid
TLC
trigonelline
description Trigonelline (N-methylnicotinate) biosynthesized from nicotinate is one of the metabolically active pyridine alkaloid, widely distributed in plant kingdom. In the present study trigonelline has been isolated from various plant parts and callus cultures of Moringa oleifera Lam., Moringaceae, and was identified using TLC, GLC, GC-MS, which was comparable to that of the standard trigonelline. The trigonelline recovery was found to be maximum in the pods and minimum in flowers. In order to enhance the production of trigonelline in vitro grown cultures, different treatment doses of nicotinic acid (250, 500 and 750 mg L-1) were supplemented in the medium as precursor. Maximum increase (up to 1.10 fold) was observed in the treatment dose of 500 mg L-1 of nicotinic acid.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000500010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000500010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2012005000041
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.22 n.5 2012
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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