Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ornamental Horticulture (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000300218 |
Resumo: | ABSTRACT Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation. |
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Ornamental Horticulture (Campinas) |
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Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)Gerbera jamesoniimicropropagationsterilization methodscost reductionchemical alterationsphytotoxicityABSTRACT Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation.Sociedade Brasileira de Floricultura e Plantas Ornamentais2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000300218Ornamental Horticulture v.24 n.3 2018reponame:Ornamental Horticulture (Campinas)instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)instacron:SBFPO10.14295/oh.v24i3.1222info:eu-repo/semantics/openAccessCardoso,Jean CarlosImthurn,Ana Carolina Petiteng2018-10-24T00:00:00Zoai:scielo:S2447-536X2018000300218Revistahttp://ornamentalhorticulture.emnuvens.com.br/rbho/indexhttps://old.scielo.br/oai/scielo-oai.php||revista.ornamentalhorticulture@gmail.com2447-536X2447-536Xopendoar:2018-10-24T00:00Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)false |
dc.title.none.fl_str_mv |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
title |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
spellingShingle |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) Cardoso,Jean Carlos Gerbera jamesonii micropropagation sterilization methods cost reduction chemical alterations phytotoxicity |
title_short |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
title_full |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
title_fullStr |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
title_full_unstemmed |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
title_sort |
Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
author |
Cardoso,Jean Carlos |
author_facet |
Cardoso,Jean Carlos Imthurn,Ana Carolina Petit |
author_role |
author |
author2 |
Imthurn,Ana Carolina Petit |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Cardoso,Jean Carlos Imthurn,Ana Carolina Petit |
dc.subject.por.fl_str_mv |
Gerbera jamesonii micropropagation sterilization methods cost reduction chemical alterations phytotoxicity |
topic |
Gerbera jamesonii micropropagation sterilization methods cost reduction chemical alterations phytotoxicity |
description |
ABSTRACT Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000300218 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000300218 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.14295/oh.v24i3.1222 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
dc.source.none.fl_str_mv |
Ornamental Horticulture v.24 n.3 2018 reponame:Ornamental Horticulture (Campinas) instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) instacron:SBFPO |
instname_str |
Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
instacron_str |
SBFPO |
institution |
SBFPO |
reponame_str |
Ornamental Horticulture (Campinas) |
collection |
Ornamental Horticulture (Campinas) |
repository.name.fl_str_mv |
Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
repository.mail.fl_str_mv |
||revista.ornamentalhorticulture@gmail.com |
_version_ |
1754734900215283712 |