Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants
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Publication Date: | 2010 |
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Format: | Article |
Language: | eng |
Source: | Revista brasileira de fruticultura (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 |
Summary: | This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated. |
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Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plantsAnanas comosus var. comosusin vitro cultureplant regulatorsnodal segmentsgermplasm conservationmicropropagationThis work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.Sociedade Brasileira de Fruticultura2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035Revista Brasileira de Fruticultura v.32 n.2 2010reponame:Revista brasileira de fruticultura (Online)instname:Sociedade Brasileira de Fruticultura (SBF)instacron:SBFRU10.1590/S0100-29452010005000075info:eu-repo/semantics/openAccessSouza,Fernanda Vidigal DuarteCanto,Ana Maria Mascarenhas EloySouza,Antônio da SilvaCosta,Maria Angélica Pereira de Carvalhoeng2010-07-22T00:00:00Zoai:scielo:S0100-29452010000200035Revistahttp://www.scielo.br/rbfhttps://old.scielo.br/oai/scielo-oai.phprbf@fcav.unesp.br||http://rbf.org.br/1806-99670100-2945opendoar:2010-07-22T00:00Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)false |
dc.title.none.fl_str_mv |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
title |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
spellingShingle |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants Souza,Fernanda Vidigal Duarte Ananas comosus var. comosus in vitro culture plant regulators nodal segments germplasm conservation micropropagation |
title_short |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
title_full |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
title_fullStr |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
title_full_unstemmed |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
title_sort |
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants |
author |
Souza,Fernanda Vidigal Duarte |
author_facet |
Souza,Fernanda Vidigal Duarte Canto,Ana Maria Mascarenhas Eloy Souza,Antônio da Silva Costa,Maria Angélica Pereira de Carvalho |
author_role |
author |
author2 |
Canto,Ana Maria Mascarenhas Eloy Souza,Antônio da Silva Costa,Maria Angélica Pereira de Carvalho |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Souza,Fernanda Vidigal Duarte Canto,Ana Maria Mascarenhas Eloy Souza,Antônio da Silva Costa,Maria Angélica Pereira de Carvalho |
dc.subject.por.fl_str_mv |
Ananas comosus var. comosus in vitro culture plant regulators nodal segments germplasm conservation micropropagation |
topic |
Ananas comosus var. comosus in vitro culture plant regulators nodal segments germplasm conservation micropropagation |
description |
This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-29452010005000075 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
dc.source.none.fl_str_mv |
Revista Brasileira de Fruticultura v.32 n.2 2010 reponame:Revista brasileira de fruticultura (Online) instname:Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU |
instname_str |
Sociedade Brasileira de Fruticultura (SBF) |
instacron_str |
SBFRU |
institution |
SBFRU |
reponame_str |
Revista brasileira de fruticultura (Online) |
collection |
Revista brasileira de fruticultura (Online) |
repository.name.fl_str_mv |
Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF) |
repository.mail.fl_str_mv |
rbf@fcav.unesp.br||http://rbf.org.br/ |
_version_ |
1752122488797528064 |