Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect

Detalhes bibliográficos
Autor(a) principal: Santos, Maria do Desterro Mendes
Data de Publicação: 2008
Outros Autores: Ribeiro, Dalva Graciano, Torres, Antonio Carlos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/547
Resumo: The objective of this work was to evaluate the effect of benzylaminopurine (BAP) additions, in combination with naphthaleneacetic acid (NAA), and of subcultures periods on in vitro shoot formation of ornamental pineapple. The basal liquid medium consisted of MS salts and vitamins. The experiment was a completely randomized design, in a factorial arrangement 6x2x4, with six concentrations of BAP (0, 0.125, 0.25, 0.5, 1, e 2 mg L-1) and two of NAA (0 and 0.1 mg L-1), and four subculturing periods (30, 60, 90 e120 days). The explants consisted of individualized shoots with 1 cm length from in vitro cultures. Shoot formation was observed in medium supplemented with BAP in all subculturing periods. The greatest averagenumber of shoots per explant was obtained with BAP concentration of 1.2 mg L-1 and 120 days of subcultive.
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spelling Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effectBrotações adventícias de abacaxizeiro ornamental sob o efeito de benzilaminopurina, ácido naftalenoacético e períodos de subcultivoAnanas comosus; in vitro culture; micropropagationAnanas comosus; cultivo in vitro; micropropagaçãoThe objective of this work was to evaluate the effect of benzylaminopurine (BAP) additions, in combination with naphthaleneacetic acid (NAA), and of subcultures periods on in vitro shoot formation of ornamental pineapple. The basal liquid medium consisted of MS salts and vitamins. The experiment was a completely randomized design, in a factorial arrangement 6x2x4, with six concentrations of BAP (0, 0.125, 0.25, 0.5, 1, e 2 mg L-1) and two of NAA (0 and 0.1 mg L-1), and four subculturing periods (30, 60, 90 e120 days). The explants consisted of individualized shoots with 1 cm length from in vitro cultures. Shoot formation was observed in medium supplemented with BAP in all subculturing periods. The greatest averagenumber of shoots per explant was obtained with BAP concentration of 1.2 mg L-1 and 120 days of subcultive.O objetivo deste trabalho foi avaliar os efeitos da adição de benzilaminopurina (BAP), em combinação com o ácido naftalenoacético (ANA), e dos períodos de subcultivo, na formação de brotações in vitro de abacaxizeiro ornamental. O meio básico líquido consistiu de sais minerais MS e vitaminas. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 6x2x4, com seis concentrações de BAP (0, 0,125, 0,25, 0,5, 1 e 2 mg L-1) e duas de ANA (0 e 0,1 mg L-1), e quatro períodos de subcultivo (30, 60, 90 e 120 dias). Os explantes consistiram de brotações individuais de 1 cm de comprimento, oriundas da cultura in vitro. A formação de novas brotações foi observada em meio suplementado com BAP, em todos os períodos de subcultivo. O maior número médio de brotos por explante foi obtido em meio com a concentração de 1,2 mg L-1 de BAP e 120 dias de subcultivo.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqSantos, Maria do Desterro MendesRibeiro, Dalva GracianoTorres, Antonio Carlos2008-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/547Pesquisa Agropecuaria Brasileira; v.43, n.9, set. 2008; 1115-1120Pesquisa Agropecuária Brasileira; v.43, n.9, set. 2008; 1115-11201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/547/5490https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/547/511https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/547/512https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/547/513info:eu-repo/semantics/openAccess2014-07-16T16:58:31Zoai:ojs.seer.sct.embrapa.br:article/547Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-16T16:58:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
Brotações adventícias de abacaxizeiro ornamental sob o efeito de benzilaminopurina, ácido naftalenoacético e períodos de subcultivo
title Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
spellingShingle Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
Santos, Maria do Desterro Mendes
Ananas comosus; in vitro culture; micropropagation
Ananas comosus; cultivo in vitro; micropropagação
title_short Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
title_full Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
title_fullStr Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
title_full_unstemmed Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
title_sort Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect
author Santos, Maria do Desterro Mendes
author_facet Santos, Maria do Desterro Mendes
Ribeiro, Dalva Graciano
Torres, Antonio Carlos
author_role author
author2 Ribeiro, Dalva Graciano
Torres, Antonio Carlos
author2_role author
author
dc.contributor.none.fl_str_mv
CNPq
dc.contributor.author.fl_str_mv Santos, Maria do Desterro Mendes
Ribeiro, Dalva Graciano
Torres, Antonio Carlos
dc.subject.por.fl_str_mv Ananas comosus; in vitro culture; micropropagation
Ananas comosus; cultivo in vitro; micropropagação
topic Ananas comosus; in vitro culture; micropropagation
Ananas comosus; cultivo in vitro; micropropagação
description The objective of this work was to evaluate the effect of benzylaminopurine (BAP) additions, in combination with naphthaleneacetic acid (NAA), and of subcultures periods on in vitro shoot formation of ornamental pineapple. The basal liquid medium consisted of MS salts and vitamins. The experiment was a completely randomized design, in a factorial arrangement 6x2x4, with six concentrations of BAP (0, 0.125, 0.25, 0.5, 1, e 2 mg L-1) and two of NAA (0 and 0.1 mg L-1), and four subculturing periods (30, 60, 90 e120 days). The explants consisted of individualized shoots with 1 cm length from in vitro cultures. Shoot formation was observed in medium supplemented with BAP in all subculturing periods. The greatest averagenumber of shoots per explant was obtained with BAP concentration of 1.2 mg L-1 and 120 days of subcultive.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-29
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/547
url https://seer.sct.embrapa.br/index.php/pab/article/view/547
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/547/5490
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/547/511
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/547/512
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/547/513
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.9, set. 2008; 1115-1120
Pesquisa Agropecuária Brasileira; v.43, n.9, set. 2008; 1115-1120
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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