Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de fruticultura (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802 |
Resumo: | ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs. |
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Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setaceaPTA-ELISAfruit hardening viruspassion fruitplant resistanceABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.Sociedade Brasileira de Fruticultura2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802Revista Brasileira de Fruticultura v.40 n.1 2018reponame:Revista brasileira de fruticultura (Online)instname:Sociedade Brasileira de Fruticultura (SBF)instacron:SBFRU10.1590/0100-29452017607info:eu-repo/semantics/openAccessSACOMAN,NAYARA NIELEVIANA,ALEXANDRE PIOCARVALHO,VIRGINIA SILVASANTOS,EILEEN AZEVEDORODRIGUES,ROSANAeng2018-02-22T00:00:00Zoai:scielo:S0100-29452018000100802Revistahttp://www.scielo.br/rbfhttps://old.scielo.br/oai/scielo-oai.phprbf@fcav.unesp.br||http://rbf.org.br/1806-99670100-2945opendoar:2018-02-22T00:00Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)false |
dc.title.none.fl_str_mv |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
title |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
spellingShingle |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea SACOMAN,NAYARA NIELE PTA-ELISA fruit hardening virus passion fruit plant resistance |
title_short |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
title_full |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
title_fullStr |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
title_full_unstemmed |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
title_sort |
Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
author |
SACOMAN,NAYARA NIELE |
author_facet |
SACOMAN,NAYARA NIELE VIANA,ALEXANDRE PIO CARVALHO,VIRGINIA SILVA SANTOS,EILEEN AZEVEDO RODRIGUES,ROSANA |
author_role |
author |
author2 |
VIANA,ALEXANDRE PIO CARVALHO,VIRGINIA SILVA SANTOS,EILEEN AZEVEDO RODRIGUES,ROSANA |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
SACOMAN,NAYARA NIELE VIANA,ALEXANDRE PIO CARVALHO,VIRGINIA SILVA SANTOS,EILEEN AZEVEDO RODRIGUES,ROSANA |
dc.subject.por.fl_str_mv |
PTA-ELISA fruit hardening virus passion fruit plant resistance |
topic |
PTA-ELISA fruit hardening virus passion fruit plant resistance |
description |
ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-29452017607 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
dc.source.none.fl_str_mv |
Revista Brasileira de Fruticultura v.40 n.1 2018 reponame:Revista brasileira de fruticultura (Online) instname:Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU |
instname_str |
Sociedade Brasileira de Fruticultura (SBF) |
instacron_str |
SBFRU |
institution |
SBFRU |
reponame_str |
Revista brasileira de fruticultura (Online) |
collection |
Revista brasileira de fruticultura (Online) |
repository.name.fl_str_mv |
Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF) |
repository.mail.fl_str_mv |
rbf@fcav.unesp.br||http://rbf.org.br/ |
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1752122494862491648 |