Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de fruticultura (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300026 |
Resumo: | The majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conserved motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highly conserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reverse primers. Forty-eight clones were sequenced from each species. The 96 sequences generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb|AAP45165.1| putative disease resistant protein RGA3 (Solanum bulbocastanum). To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases. |
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Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort familyCaricaceaenucleotide-binding site (NBS)V. caulifloraC. papayapapayaThe majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conserved motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highly conserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reverse primers. Forty-eight clones were sequenced from each species. The 96 sequences generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb|AAP45165.1| putative disease resistant protein RGA3 (Solanum bulbocastanum). To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases.Sociedade Brasileira de Fruticultura2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300026Revista Brasileira de Fruticultura v.28 n.3 2006reponame:Revista brasileira de fruticultura (Online)instname:Sociedade Brasileira de Fruticultura (SBF)instacron:SBFRU10.1590/S0100-29452006000300026info:eu-repo/semantics/openAccessAmaral,Paulo de Paiva RosaAlves,Paulo César MartinsMartins,Natália FlorêncioSilva,Felipe Rodrigues daCapdeville,Guy deSouza Júnior,Manoel Teixeiraeng2007-02-28T00:00:00Zoai:scielo:S0100-29452006000300026Revistahttp://www.scielo.br/rbfhttps://old.scielo.br/oai/scielo-oai.phprbf@fcav.unesp.br||http://rbf.org.br/1806-99670100-2945opendoar:2007-02-28T00:00Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)false |
dc.title.none.fl_str_mv |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
title |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
spellingShingle |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family Amaral,Paulo de Paiva Rosa Caricaceae nucleotide-binding site (NBS) V. cauliflora C. papaya papaya |
title_short |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
title_full |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
title_fullStr |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
title_full_unstemmed |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
title_sort |
Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family |
author |
Amaral,Paulo de Paiva Rosa |
author_facet |
Amaral,Paulo de Paiva Rosa Alves,Paulo César Martins Martins,Natália Florêncio Silva,Felipe Rodrigues da Capdeville,Guy de Souza Júnior,Manoel Teixeira |
author_role |
author |
author2 |
Alves,Paulo César Martins Martins,Natália Florêncio Silva,Felipe Rodrigues da Capdeville,Guy de Souza Júnior,Manoel Teixeira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Amaral,Paulo de Paiva Rosa Alves,Paulo César Martins Martins,Natália Florêncio Silva,Felipe Rodrigues da Capdeville,Guy de Souza Júnior,Manoel Teixeira |
dc.subject.por.fl_str_mv |
Caricaceae nucleotide-binding site (NBS) V. cauliflora C. papaya papaya |
topic |
Caricaceae nucleotide-binding site (NBS) V. cauliflora C. papaya papaya |
description |
The majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conserved motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highly conserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reverse primers. Forty-eight clones were sequenced from each species. The 96 sequences generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb|AAP45165.1| putative disease resistant protein RGA3 (Solanum bulbocastanum). To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300026 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-29452006000300026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
dc.source.none.fl_str_mv |
Revista Brasileira de Fruticultura v.28 n.3 2006 reponame:Revista brasileira de fruticultura (Online) instname:Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU |
instname_str |
Sociedade Brasileira de Fruticultura (SBF) |
instacron_str |
SBFRU |
institution |
SBFRU |
reponame_str |
Revista brasileira de fruticultura (Online) |
collection |
Revista brasileira de fruticultura (Online) |
repository.name.fl_str_mv |
Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF) |
repository.mail.fl_str_mv |
rbf@fcav.unesp.br||http://rbf.org.br/ |
_version_ |
1752122485200912384 |