In vitro organogenesis and genetic transformation of mandarin cultivars

Detalhes bibliográficos
Autor(a) principal: Soriano,Leonardo
Data de Publicação: 2019
Outros Autores: Tavano,Eveline Carla da Rocha, Correa,Marcelo Favaretto, Harakava,Ricardo, Mendes,Beatriz Madalena Januzzi, Mourão Filho,Francisco de Assis Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de fruticultura (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802
Resumo: Abstract The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.
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spelling In vitro organogenesis and genetic transformation of mandarin cultivarsantimicrobial peptidecultivar improvementcytokininhuanglongbingtransgenic plantsAbstract The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.Sociedade Brasileira de Fruticultura2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802Revista Brasileira de Fruticultura v.41 n.2 2019reponame:Revista brasileira de fruticultura (Online)instname:Sociedade Brasileira de Fruticultura (SBF)instacron:SBFRU10.1590/0100-29452019116info:eu-repo/semantics/openAccessSoriano,LeonardoTavano,Eveline Carla da RochaCorrea,Marcelo FavarettoHarakava,RicardoMendes,Beatriz Madalena JanuzziMourão Filho,Francisco de Assis Alveseng2019-04-16T00:00:00Zoai:scielo:S0100-29452019000200802Revistahttp://www.scielo.br/rbfhttps://old.scielo.br/oai/scielo-oai.phprbf@fcav.unesp.br||http://rbf.org.br/1806-99670100-2945opendoar:2019-04-16T00:00Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)false
dc.title.none.fl_str_mv In vitro organogenesis and genetic transformation of mandarin cultivars
title In vitro organogenesis and genetic transformation of mandarin cultivars
spellingShingle In vitro organogenesis and genetic transformation of mandarin cultivars
Soriano,Leonardo
antimicrobial peptide
cultivar improvement
cytokinin
huanglongbing
transgenic plants
title_short In vitro organogenesis and genetic transformation of mandarin cultivars
title_full In vitro organogenesis and genetic transformation of mandarin cultivars
title_fullStr In vitro organogenesis and genetic transformation of mandarin cultivars
title_full_unstemmed In vitro organogenesis and genetic transformation of mandarin cultivars
title_sort In vitro organogenesis and genetic transformation of mandarin cultivars
author Soriano,Leonardo
author_facet Soriano,Leonardo
Tavano,Eveline Carla da Rocha
Correa,Marcelo Favaretto
Harakava,Ricardo
Mendes,Beatriz Madalena Januzzi
Mourão Filho,Francisco de Assis Alves
author_role author
author2 Tavano,Eveline Carla da Rocha
Correa,Marcelo Favaretto
Harakava,Ricardo
Mendes,Beatriz Madalena Januzzi
Mourão Filho,Francisco de Assis Alves
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Soriano,Leonardo
Tavano,Eveline Carla da Rocha
Correa,Marcelo Favaretto
Harakava,Ricardo
Mendes,Beatriz Madalena Januzzi
Mourão Filho,Francisco de Assis Alves
dc.subject.por.fl_str_mv antimicrobial peptide
cultivar improvement
cytokinin
huanglongbing
transgenic plants
topic antimicrobial peptide
cultivar improvement
cytokinin
huanglongbing
transgenic plants
description Abstract The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0100-29452019116
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fruticultura
publisher.none.fl_str_mv Sociedade Brasileira de Fruticultura
dc.source.none.fl_str_mv Revista Brasileira de Fruticultura v.41 n.2 2019
reponame:Revista brasileira de fruticultura (Online)
instname:Sociedade Brasileira de Fruticultura (SBF)
instacron:SBFRU
instname_str Sociedade Brasileira de Fruticultura (SBF)
instacron_str SBFRU
institution SBFRU
reponame_str Revista brasileira de fruticultura (Online)
collection Revista brasileira de fruticultura (Online)
repository.name.fl_str_mv Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)
repository.mail.fl_str_mv rbf@fcav.unesp.br||http://rbf.org.br/
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