Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de fruticultura (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452022000200901 |
Resumo: | Abstract The best conditions for in vitro sucrose hydrolysis based on invertase from Candida guilliermondii (ICg) were studied and the kinetic parameters KM,Vmax, and thermal stability of ICg were determined. Candida guilliermondii (Cg) yeast isolated and lyophilized from peach solid wastes was identified using the API 20C AUX method. Subsequently, the Cg was submitted to an autolysis process using NaHCO3 at 200 mM under 200 rpm stirring and 40 °C for 24 h. The enzyme extracts obtained were recovered through precipitation with acetone followed by dialysis and ion-exchange chromatography. The extract purified through precipitation with acetone had activity of 27.7 U.mg-1 and 56% recovery whereas the chromatography process yielded 46.5 U.mg-1 and 44.8%. The optimal sucrose hydrolysis conditions were pH 5.0 and 50 °C, resulting in KM of 30.5 mM and 28.7 mM sucrose, respectively, at 25 °C and 50 °C, both with Michaelian behavior. Thermal inactivation of ICg exhibited first-order apparent kinetics and its residual activity was typically linear between 40 °C and 70 °C. Three isoenzymes were detected through electrophoresis. |
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Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastesYeastautolysisisoenzymesinverted sugarAbstract The best conditions for in vitro sucrose hydrolysis based on invertase from Candida guilliermondii (ICg) were studied and the kinetic parameters KM,Vmax, and thermal stability of ICg were determined. Candida guilliermondii (Cg) yeast isolated and lyophilized from peach solid wastes was identified using the API 20C AUX method. Subsequently, the Cg was submitted to an autolysis process using NaHCO3 at 200 mM under 200 rpm stirring and 40 °C for 24 h. The enzyme extracts obtained were recovered through precipitation with acetone followed by dialysis and ion-exchange chromatography. The extract purified through precipitation with acetone had activity of 27.7 U.mg-1 and 56% recovery whereas the chromatography process yielded 46.5 U.mg-1 and 44.8%. The optimal sucrose hydrolysis conditions were pH 5.0 and 50 °C, resulting in KM of 30.5 mM and 28.7 mM sucrose, respectively, at 25 °C and 50 °C, both with Michaelian behavior. Thermal inactivation of ICg exhibited first-order apparent kinetics and its residual activity was typically linear between 40 °C and 70 °C. Three isoenzymes were detected through electrophoresis.Sociedade Brasileira de Fruticultura2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452022000200901Revista Brasileira de Fruticultura v.44 n.2 2022reponame:Revista brasileira de fruticultura (Online)instname:Sociedade Brasileira de Fruticultura (SBF)instacron:SBFRU10.1590/0100-29452022849info:eu-repo/semantics/openAccessAvila,Tariani LemosToralles,Ricardo PeraçaJansen,Estefani TavaresFerreira,Marcela VegaKuhn,Claudio RafaelRuiz,Walter Augustoeng2022-03-15T00:00:00Zoai:scielo:S0100-29452022000200901Revistahttp://www.scielo.br/rbfhttps://old.scielo.br/oai/scielo-oai.phprbf@fcav.unesp.br||http://rbf.org.br/1806-99670100-2945opendoar:2022-03-15T00:00Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)false |
dc.title.none.fl_str_mv |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
title |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
spellingShingle |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes Avila,Tariani Lemos Yeast autolysis isoenzymes inverted sugar |
title_short |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
title_full |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
title_fullStr |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
title_full_unstemmed |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
title_sort |
Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes |
author |
Avila,Tariani Lemos |
author_facet |
Avila,Tariani Lemos Toralles,Ricardo Peraça Jansen,Estefani Tavares Ferreira,Marcela Vega Kuhn,Claudio Rafael Ruiz,Walter Augusto |
author_role |
author |
author2 |
Toralles,Ricardo Peraça Jansen,Estefani Tavares Ferreira,Marcela Vega Kuhn,Claudio Rafael Ruiz,Walter Augusto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Avila,Tariani Lemos Toralles,Ricardo Peraça Jansen,Estefani Tavares Ferreira,Marcela Vega Kuhn,Claudio Rafael Ruiz,Walter Augusto |
dc.subject.por.fl_str_mv |
Yeast autolysis isoenzymes inverted sugar |
topic |
Yeast autolysis isoenzymes inverted sugar |
description |
Abstract The best conditions for in vitro sucrose hydrolysis based on invertase from Candida guilliermondii (ICg) were studied and the kinetic parameters KM,Vmax, and thermal stability of ICg were determined. Candida guilliermondii (Cg) yeast isolated and lyophilized from peach solid wastes was identified using the API 20C AUX method. Subsequently, the Cg was submitted to an autolysis process using NaHCO3 at 200 mM under 200 rpm stirring and 40 °C for 24 h. The enzyme extracts obtained were recovered through precipitation with acetone followed by dialysis and ion-exchange chromatography. The extract purified through precipitation with acetone had activity of 27.7 U.mg-1 and 56% recovery whereas the chromatography process yielded 46.5 U.mg-1 and 44.8%. The optimal sucrose hydrolysis conditions were pH 5.0 and 50 °C, resulting in KM of 30.5 mM and 28.7 mM sucrose, respectively, at 25 °C and 50 °C, both with Michaelian behavior. Thermal inactivation of ICg exhibited first-order apparent kinetics and its residual activity was typically linear between 40 °C and 70 °C. Three isoenzymes were detected through electrophoresis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452022000200901 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452022000200901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-29452022849 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
dc.source.none.fl_str_mv |
Revista Brasileira de Fruticultura v.44 n.2 2022 reponame:Revista brasileira de fruticultura (Online) instname:Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU |
instname_str |
Sociedade Brasileira de Fruticultura (SBF) |
instacron_str |
SBFRU |
institution |
SBFRU |
reponame_str |
Revista brasileira de fruticultura (Online) |
collection |
Revista brasileira de fruticultura (Online) |
repository.name.fl_str_mv |
Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF) |
repository.mail.fl_str_mv |
rbf@fcav.unesp.br||http://rbf.org.br/ |
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1752122496780337152 |