Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Fisiologia Vegetal (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100001 |
Resumo: | Leaf lipoxygenases (LOX) are involved with important physiological processes such as plant growth and development, senescence, biosynthesis of regulatory molecules, and response to pathogens and insects. We did a biochemical evaluation of the LOX pathway of soybean leaves submitted to wounding in a normal genotype (IAC-100) and its counterpart lacking seed LOX (IAC-100 TN). Our results indicate that LOX activities in the different pHs and temperatures tended to be higher in the wounded plants compared to their respective controls. The K M app values at 168 h after wounding reached a minimum in both genotypes indicating that the plants respond by changing the leaf LOX pool. There was an increase on protease inhibitor levels in all time points after wounding, for both cultivars. The levels of hexanal and total aldehydes are similar for the wounded plants at different times after wounding and their respective controls for both genotypes. Our results strongly suggest that the LOX pathway is activated during the wound response leading to jasmonate by the initial action of hydroperoxide cyclase. In addition, the results show that the genetic removal of seed LOX does not interfere with the plants ability to respond to wound via the LOX pathway. |
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Revista Brasileira de Fisiologia Vegetal (Online) |
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Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to woundingLipoxygenasessoybeanmechanical woundingLeaf lipoxygenases (LOX) are involved with important physiological processes such as plant growth and development, senescence, biosynthesis of regulatory molecules, and response to pathogens and insects. We did a biochemical evaluation of the LOX pathway of soybean leaves submitted to wounding in a normal genotype (IAC-100) and its counterpart lacking seed LOX (IAC-100 TN). Our results indicate that LOX activities in the different pHs and temperatures tended to be higher in the wounded plants compared to their respective controls. The K M app values at 168 h after wounding reached a minimum in both genotypes indicating that the plants respond by changing the leaf LOX pool. There was an increase on protease inhibitor levels in all time points after wounding, for both cultivars. The levels of hexanal and total aldehydes are similar for the wounded plants at different times after wounding and their respective controls for both genotypes. Our results strongly suggest that the LOX pathway is activated during the wound response leading to jasmonate by the initial action of hydroperoxide cyclase. In addition, the results show that the genetic removal of seed LOX does not interfere with the plants ability to respond to wound via the LOX pathway.Sociedade Brasileira de Fisiologia Vegetal2001-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100001Revista Brasileira de Fisiologia Vegetal v.13 n.1 2001reponame:Revista Brasileira de Fisiologia Vegetal (Online)instname:Sociedade Brasileira de Fisiologia Vegetal (SBFV)instacron:SBFV10.1590/S0103-31312001000100001info:eu-repo/semantics/openAccessVIEIRA,ANGÉLICA APARECIDAOLIVEIRA,MARIA GORETI DE ALMEIDAJOSÉ,INES CHAMELPIOVESAN,NEWTON DENIZREZENDE,SEBASTIÃO TAVARES DEMOREIRA,MAURILIO ALVESBARROS,EVERALDO GONÇALVES DEeng2002-11-20T00:00:00Zoai:scielo:S0103-31312001000100001Revistahttps://www.scielo.br/j/rbfv/ONGhttps://old.scielo.br/oai/scielo-oai.phppmazza@unicamp.br1806-93550103-3131opendoar:2002-11-20T00:00Revista Brasileira de Fisiologia Vegetal (Online) - Sociedade Brasileira de Fisiologia Vegetal (SBFV)false |
dc.title.none.fl_str_mv |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
title |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
spellingShingle |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding VIEIRA,ANGÉLICA APARECIDA Lipoxygenases soybean mechanical wounding |
title_short |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
title_full |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
title_fullStr |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
title_full_unstemmed |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
title_sort |
Biochemical evaluation of lipoxygenase pathway of soybean plants submitted to wounding |
author |
VIEIRA,ANGÉLICA APARECIDA |
author_facet |
VIEIRA,ANGÉLICA APARECIDA OLIVEIRA,MARIA GORETI DE ALMEIDA JOSÉ,INES CHAMEL PIOVESAN,NEWTON DENIZ REZENDE,SEBASTIÃO TAVARES DE MOREIRA,MAURILIO ALVES BARROS,EVERALDO GONÇALVES DE |
author_role |
author |
author2 |
OLIVEIRA,MARIA GORETI DE ALMEIDA JOSÉ,INES CHAMEL PIOVESAN,NEWTON DENIZ REZENDE,SEBASTIÃO TAVARES DE MOREIRA,MAURILIO ALVES BARROS,EVERALDO GONÇALVES DE |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
VIEIRA,ANGÉLICA APARECIDA OLIVEIRA,MARIA GORETI DE ALMEIDA JOSÉ,INES CHAMEL PIOVESAN,NEWTON DENIZ REZENDE,SEBASTIÃO TAVARES DE MOREIRA,MAURILIO ALVES BARROS,EVERALDO GONÇALVES DE |
dc.subject.por.fl_str_mv |
Lipoxygenases soybean mechanical wounding |
topic |
Lipoxygenases soybean mechanical wounding |
description |
Leaf lipoxygenases (LOX) are involved with important physiological processes such as plant growth and development, senescence, biosynthesis of regulatory molecules, and response to pathogens and insects. We did a biochemical evaluation of the LOX pathway of soybean leaves submitted to wounding in a normal genotype (IAC-100) and its counterpart lacking seed LOX (IAC-100 TN). Our results indicate that LOX activities in the different pHs and temperatures tended to be higher in the wounded plants compared to their respective controls. The K M app values at 168 h after wounding reached a minimum in both genotypes indicating that the plants respond by changing the leaf LOX pool. There was an increase on protease inhibitor levels in all time points after wounding, for both cultivars. The levels of hexanal and total aldehydes are similar for the wounded plants at different times after wounding and their respective controls for both genotypes. Our results strongly suggest that the LOX pathway is activated during the wound response leading to jasmonate by the initial action of hydroperoxide cyclase. In addition, the results show that the genetic removal of seed LOX does not interfere with the plants ability to respond to wound via the LOX pathway. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-31312001000100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fisiologia Vegetal |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fisiologia Vegetal |
dc.source.none.fl_str_mv |
Revista Brasileira de Fisiologia Vegetal v.13 n.1 2001 reponame:Revista Brasileira de Fisiologia Vegetal (Online) instname:Sociedade Brasileira de Fisiologia Vegetal (SBFV) instacron:SBFV |
instname_str |
Sociedade Brasileira de Fisiologia Vegetal (SBFV) |
instacron_str |
SBFV |
institution |
SBFV |
reponame_str |
Revista Brasileira de Fisiologia Vegetal (Online) |
collection |
Revista Brasileira de Fisiologia Vegetal (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Fisiologia Vegetal (Online) - Sociedade Brasileira de Fisiologia Vegetal (SBFV) |
repository.mail.fl_str_mv |
pmazza@unicamp.br |
_version_ |
1754820904251031552 |