A rapid alternative technique for obtaining silver-positive patterns in chromosomes

Detalhes bibliográficos
Autor(a) principal: Kavalco,Karine Frehner
Data de Publicação: 2004
Outros Autores: Pazza,Rubens
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
Resumo: Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
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spelling A rapid alternative technique for obtaining silver-positive patterns in chromosomessilver stainingAg-NORmicrowaveschromosome bandingSilver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012Genetics and Molecular Biology v.27 n.2 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000200012info:eu-repo/semantics/openAccessKavalco,Karine FrehnerPazza,Rubenseng2004-07-20T00:00:00Zoai:scielo:S1415-47572004000200012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-07-20T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv A rapid alternative technique for obtaining silver-positive patterns in chromosomes
title A rapid alternative technique for obtaining silver-positive patterns in chromosomes
spellingShingle A rapid alternative technique for obtaining silver-positive patterns in chromosomes
Kavalco,Karine Frehner
silver staining
Ag-NOR
microwaves
chromosome banding
title_short A rapid alternative technique for obtaining silver-positive patterns in chromosomes
title_full A rapid alternative technique for obtaining silver-positive patterns in chromosomes
title_fullStr A rapid alternative technique for obtaining silver-positive patterns in chromosomes
title_full_unstemmed A rapid alternative technique for obtaining silver-positive patterns in chromosomes
title_sort A rapid alternative technique for obtaining silver-positive patterns in chromosomes
author Kavalco,Karine Frehner
author_facet Kavalco,Karine Frehner
Pazza,Rubens
author_role author
author2 Pazza,Rubens
author2_role author
dc.contributor.author.fl_str_mv Kavalco,Karine Frehner
Pazza,Rubens
dc.subject.por.fl_str_mv silver staining
Ag-NOR
microwaves
chromosome banding
topic silver staining
Ag-NOR
microwaves
chromosome banding
description Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572004000200012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.27 n.2 2004
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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