Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector

Detalhes bibliográficos
Autor(a) principal: Rudi Emerson de Lima,Procópio
Data de Publicação: 2011
Outros Autores: Araújo,Welington Luiz, Andreote,Fernando Dini, Azevedo,João Lúcio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018
Resumo: A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants.
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spelling Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vectorgreen fluorescent proteinbacteriahost plantEucalyptusA circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants.Sociedade Brasileira de Genética2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018Genetics and Molecular Biology v.34 n.1 2011reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000096info:eu-repo/semantics/openAccessRudi Emerson de Lima,ProcópioAraújo,Welington LuizAndreote,Fernando DiniAzevedo,João Lúcioeng2011-01-28T00:00:00Zoai:scielo:S1415-47572011000100018Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2011-01-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
title Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
spellingShingle Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
Rudi Emerson de Lima,Procópio
green fluorescent protein
bacteria
host plant
Eucalyptus
title_short Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
title_full Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
title_fullStr Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
title_full_unstemmed Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
title_sort Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
author Rudi Emerson de Lima,Procópio
author_facet Rudi Emerson de Lima,Procópio
Araújo,Welington Luiz
Andreote,Fernando Dini
Azevedo,João Lúcio
author_role author
author2 Araújo,Welington Luiz
Andreote,Fernando Dini
Azevedo,João Lúcio
author2_role author
author
author
dc.contributor.author.fl_str_mv Rudi Emerson de Lima,Procópio
Araújo,Welington Luiz
Andreote,Fernando Dini
Azevedo,João Lúcio
dc.subject.por.fl_str_mv green fluorescent protein
bacteria
host plant
Eucalyptus
topic green fluorescent protein
bacteria
host plant
Eucalyptus
description A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572010005000096
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.34 n.1 2011
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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