Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021 |
Resumo: | Eleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although generally the dinucleotide sequence repeat was preserved for most loci, there were significant differences in sequences interspersed within the repeat domain. This result suggested that it may be possible to unequivocally identify the geographical origin of the cultivar by inspection of the DNA sequences of the repeat region. |
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Genetics and Molecular Biology |
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Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studieswheatBraziliancultivarsDNASTRpolymorphismEleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although generally the dinucleotide sequence repeat was preserved for most loci, there were significant differences in sequences interspersed within the repeat domain. This result suggested that it may be possible to unequivocally identify the geographical origin of the cultivar by inspection of the DNA sequences of the repeat region.Sociedade Brasileira de Genética2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021Genetics and Molecular Biology v.26 n.3 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000300021info:eu-repo/semantics/openAccessLima,Vitor Lopes de AbreuSeki,Homiko AbreuRumjanek,Franklin Davideng2003-09-29T00:00:00Zoai:scielo:S1415-47572003000300021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-09-29T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
title |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
spellingShingle |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies Lima,Vitor Lopes de Abreu wheat Brazilian cultivars DNA STR polymorphism |
title_short |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
title_full |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
title_fullStr |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
title_full_unstemmed |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
title_sort |
Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies |
author |
Lima,Vitor Lopes de Abreu |
author_facet |
Lima,Vitor Lopes de Abreu Seki,Homiko Abreu Rumjanek,Franklin David |
author_role |
author |
author2 |
Seki,Homiko Abreu Rumjanek,Franklin David |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Lima,Vitor Lopes de Abreu Seki,Homiko Abreu Rumjanek,Franklin David |
dc.subject.por.fl_str_mv |
wheat Brazilian cultivars DNA STR polymorphism |
topic |
wheat Brazilian cultivars DNA STR polymorphism |
description |
Eleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although generally the dinucleotide sequence repeat was preserved for most loci, there were significant differences in sequences interspersed within the repeat domain. This result suggested that it may be possible to unequivocally identify the geographical origin of the cultivar by inspection of the DNA sequences of the repeat region. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572003000300021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.26 n.3 2003 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122378668736512 |