A shortcut in phage screening technique

Detalhes bibliográficos
Autor(a) principal: Andrade,Alexandre de
Data de Publicação: 2005
Outros Autores: Teixeira,Paula Rezende, Siviero,Fábio, Santelli,Roberto Vicente
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100025
Resumo: A simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.
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spelling A shortcut in phage screening techniquephage screeningshortcut for Benton & Davis techniquesingle agar-plate/nylon filterA simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.Sociedade Brasileira de Genética2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100025Genetics and Molecular Biology v.28 n.1 2005reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572005000100025info:eu-repo/semantics/openAccessAndrade,Alexandre deTeixeira,Paula RezendeSiviero,FábioSantelli,Roberto Vicenteeng2005-09-08T00:00:00Zoai:scielo:S1415-47572005000100025Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2005-09-08T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv A shortcut in phage screening technique
title A shortcut in phage screening technique
spellingShingle A shortcut in phage screening technique
Andrade,Alexandre de
phage screening
shortcut for Benton & Davis technique
single agar-plate/nylon filter
title_short A shortcut in phage screening technique
title_full A shortcut in phage screening technique
title_fullStr A shortcut in phage screening technique
title_full_unstemmed A shortcut in phage screening technique
title_sort A shortcut in phage screening technique
author Andrade,Alexandre de
author_facet Andrade,Alexandre de
Teixeira,Paula Rezende
Siviero,Fábio
Santelli,Roberto Vicente
author_role author
author2 Teixeira,Paula Rezende
Siviero,Fábio
Santelli,Roberto Vicente
author2_role author
author
author
dc.contributor.author.fl_str_mv Andrade,Alexandre de
Teixeira,Paula Rezende
Siviero,Fábio
Santelli,Roberto Vicente
dc.subject.por.fl_str_mv phage screening
shortcut for Benton & Davis technique
single agar-plate/nylon filter
topic phage screening
shortcut for Benton & Davis technique
single agar-plate/nylon filter
description A simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572005000100025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.28 n.1 2005
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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