PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach

Detalhes bibliográficos
Autor(a) principal: Campos,Magnólia A.
Data de Publicação: 2007
Outros Autores: Rosa,Daniel D., Teixeira,Juliana Érika C., Targon,Maria Luisa P.N., Souza,Alessandra A., Paiva,Luciano V., Stach-Machado,Dagmar R., Machado,Marcos A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020
Resumo: In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold higher in pathogen-infected than in uninfected organs, even though the differential expression profiles displayed have been quite diverse among studied species and organs. Furthermore, an insight into some contigs from four PRlgf pointed out putative members of multigene families. They appear to be evolutionarily conserved within citrus species and/or organ- or stress-specifically expressed. Our results represent a starting point regarding the extent of expression pattern differences underlying PRlgf expression and reveal genes that may prove to be useful in studies regarding biotechnological approaches or citrus resistance markers.
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spelling PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approachcitrusfunctional genomeestsgene expression profilespathogenesis-related proteinsdefense genesIn silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold higher in pathogen-infected than in uninfected organs, even though the differential expression profiles displayed have been quite diverse among studied species and organs. Furthermore, an insight into some contigs from four PRlgf pointed out putative members of multigene families. They appear to be evolutionarily conserved within citrus species and/or organ- or stress-specifically expressed. Our results represent a starting point regarding the extent of expression pattern differences underlying PRlgf expression and reveal genes that may prove to be useful in studies regarding biotechnological approaches or citrus resistance markers.Sociedade Brasileira de Genética2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020Genetics and Molecular Biology v.30 n.3 suppl.0 2007reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572007000500020info:eu-repo/semantics/openAccessCampos,Magnólia A.Rosa,Daniel D.Teixeira,Juliana Érika C.Targon,Maria Luisa P.N.Souza,Alessandra A.Paiva,Luciano V.Stach-Machado,Dagmar R.Machado,Marcos A.eng2007-11-26T00:00:00Zoai:scielo:S1415-47572007000500020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2007-11-26T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
title PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
spellingShingle PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
Campos,Magnólia A.
citrus
functional genome
ests
gene expression profiles
pathogenesis-related proteins
defense genes
title_short PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
title_full PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
title_fullStr PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
title_full_unstemmed PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
title_sort PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
author Campos,Magnólia A.
author_facet Campos,Magnólia A.
Rosa,Daniel D.
Teixeira,Juliana Érika C.
Targon,Maria Luisa P.N.
Souza,Alessandra A.
Paiva,Luciano V.
Stach-Machado,Dagmar R.
Machado,Marcos A.
author_role author
author2 Rosa,Daniel D.
Teixeira,Juliana Érika C.
Targon,Maria Luisa P.N.
Souza,Alessandra A.
Paiva,Luciano V.
Stach-Machado,Dagmar R.
Machado,Marcos A.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campos,Magnólia A.
Rosa,Daniel D.
Teixeira,Juliana Érika C.
Targon,Maria Luisa P.N.
Souza,Alessandra A.
Paiva,Luciano V.
Stach-Machado,Dagmar R.
Machado,Marcos A.
dc.subject.por.fl_str_mv citrus
functional genome
ests
gene expression profiles
pathogenesis-related proteins
defense genes
topic citrus
functional genome
ests
gene expression profiles
pathogenesis-related proteins
defense genes
description In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold higher in pathogen-infected than in uninfected organs, even though the differential expression profiles displayed have been quite diverse among studied species and organs. Furthermore, an insight into some contigs from four PRlgf pointed out putative members of multigene families. They appear to be evolutionarily conserved within citrus species and/or organ- or stress-specifically expressed. Our results represent a starting point regarding the extent of expression pattern differences underlying PRlgf expression and reveal genes that may prove to be useful in studies regarding biotechnological approaches or citrus resistance markers.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572007000500020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.30 n.3 suppl.0 2007
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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