Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019 |
Resumo: | Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species. |
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Genetics and Molecular Biology |
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Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytesthyroxinehuman lymphocyteschromosome aberrationsmitotic indexROSThyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.Sociedade Brasileira de Genética2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019Genetics and Molecular Biology v.30 n.4 2007reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572007000600019info:eu-repo/semantics/openAccessDjelic,NinoslavDjelic,DijanaSpremo-Potparevic,BiljanaZivkovic,LadaMarkovic,BiljanaLozance,OliveraBlagojevic,Miloseng2007-12-13T00:00:00Zoai:scielo:S1415-47572007000600019Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2007-12-13T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
title |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
spellingShingle |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes Djelic,Ninoslav thyroxine human lymphocytes chromosome aberrations mitotic index ROS |
title_short |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
title_full |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
title_fullStr |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
title_full_unstemmed |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
title_sort |
Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes |
author |
Djelic,Ninoslav |
author_facet |
Djelic,Ninoslav Djelic,Dijana Spremo-Potparevic,Biljana Zivkovic,Lada Markovic,Biljana Lozance,Olivera Blagojevic,Milos |
author_role |
author |
author2 |
Djelic,Dijana Spremo-Potparevic,Biljana Zivkovic,Lada Markovic,Biljana Lozance,Olivera Blagojevic,Milos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Djelic,Ninoslav Djelic,Dijana Spremo-Potparevic,Biljana Zivkovic,Lada Markovic,Biljana Lozance,Olivera Blagojevic,Milos |
dc.subject.por.fl_str_mv |
thyroxine human lymphocytes chromosome aberrations mitotic index ROS |
topic |
thyroxine human lymphocytes chromosome aberrations mitotic index ROS |
description |
Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572007000600019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.30 n.4 2007 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122381034323968 |