Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400702 |
Resumo: | Abstract Cryptococcus neoformans and Cryptococcus gattii are the etiological agents of cryptococcosis, a high mortality disease. The development of such disease depends on the interaction of fungal cells with macrophages, in which they can reside and replicate. In order to dissect the molecular mechanisms by which cryptococcal cells modulate the activity of macrophages, a genome-scale comparative analysis of transcriptional changes in macrophages exposed to Cryptococcus spp. was conducted. Altered expression of nearly 40 genes was detected in macrophages exposed to cryptococcal cells. The major processes were associated with the mTOR pathway, whose associated genes exhibited decreased expression in macrophages incubated with cryptococcal cells. Phosphorylation of p70S6K and GSK-3β was also decreased in macrophages incubated with fungal cells. In this way, Cryptococci presence could drive the modulation of mTOR pathway in macrophages possibly to increase the survival of the pathogen. |
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Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cellsMacrophagemTOR pathwayCryptococcusRNAseqinteratomic networksAbstract Cryptococcus neoformans and Cryptococcus gattii are the etiological agents of cryptococcosis, a high mortality disease. The development of such disease depends on the interaction of fungal cells with macrophages, in which they can reside and replicate. In order to dissect the molecular mechanisms by which cryptococcal cells modulate the activity of macrophages, a genome-scale comparative analysis of transcriptional changes in macrophages exposed to Cryptococcus spp. was conducted. Altered expression of nearly 40 genes was detected in macrophages exposed to cryptococcal cells. The major processes were associated with the mTOR pathway, whose associated genes exhibited decreased expression in macrophages incubated with cryptococcal cells. Phosphorylation of p70S6K and GSK-3β was also decreased in macrophages incubated with fungal cells. In this way, Cryptococci presence could drive the modulation of mTOR pathway in macrophages possibly to increase the survival of the pathogen.Sociedade Brasileira de Genética2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400702Genetics and Molecular Biology v.44 n.3 2021reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2020-0390info:eu-repo/semantics/openAccessPiffer,Alícia C.Santos,Francine M. dosThomé,Marcos P.Diehl,CamilaGarcia,Ane Wichine AcostaKinskovski,Uriel PerinSchneider,Rafael de OliveiraGerber,AlexandraFeltes,Bruno CésarSchrank,AugustoVasconcelos,Ana Tereza R.Lenz,GuidoKmetzsch,LíviaVainstein,Marilene H.Staats,Charley C.eng2021-07-30T00:00:00Zoai:scielo:S1415-47572021000400702Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2021-07-30T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
title |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
spellingShingle |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells Piffer,Alícia C. Macrophage mTOR pathway Cryptococcus RNAseq interatomic networks |
title_short |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
title_full |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
title_fullStr |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
title_full_unstemmed |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
title_sort |
Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells |
author |
Piffer,Alícia C. |
author_facet |
Piffer,Alícia C. Santos,Francine M. dos Thomé,Marcos P. Diehl,Camila Garcia,Ane Wichine Acosta Kinskovski,Uriel Perin Schneider,Rafael de Oliveira Gerber,Alexandra Feltes,Bruno César Schrank,Augusto Vasconcelos,Ana Tereza R. Lenz,Guido Kmetzsch,Lívia Vainstein,Marilene H. Staats,Charley C. |
author_role |
author |
author2 |
Santos,Francine M. dos Thomé,Marcos P. Diehl,Camila Garcia,Ane Wichine Acosta Kinskovski,Uriel Perin Schneider,Rafael de Oliveira Gerber,Alexandra Feltes,Bruno César Schrank,Augusto Vasconcelos,Ana Tereza R. Lenz,Guido Kmetzsch,Lívia Vainstein,Marilene H. Staats,Charley C. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Piffer,Alícia C. Santos,Francine M. dos Thomé,Marcos P. Diehl,Camila Garcia,Ane Wichine Acosta Kinskovski,Uriel Perin Schneider,Rafael de Oliveira Gerber,Alexandra Feltes,Bruno César Schrank,Augusto Vasconcelos,Ana Tereza R. Lenz,Guido Kmetzsch,Lívia Vainstein,Marilene H. Staats,Charley C. |
dc.subject.por.fl_str_mv |
Macrophage mTOR pathway Cryptococcus RNAseq interatomic networks |
topic |
Macrophage mTOR pathway Cryptococcus RNAseq interatomic networks |
description |
Abstract Cryptococcus neoformans and Cryptococcus gattii are the etiological agents of cryptococcosis, a high mortality disease. The development of such disease depends on the interaction of fungal cells with macrophages, in which they can reside and replicate. In order to dissect the molecular mechanisms by which cryptococcal cells modulate the activity of macrophages, a genome-scale comparative analysis of transcriptional changes in macrophages exposed to Cryptococcus spp. was conducted. Altered expression of nearly 40 genes was detected in macrophages exposed to cryptococcal cells. The major processes were associated with the mTOR pathway, whose associated genes exhibited decreased expression in macrophages incubated with cryptococcal cells. Phosphorylation of p70S6K and GSK-3β was also decreased in macrophages incubated with fungal cells. In this way, Cryptococci presence could drive the modulation of mTOR pathway in macrophages possibly to increase the survival of the pathogen. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400702 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400702 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2020-0390 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.44 n.3 2021 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122390548054016 |