Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200014 |
Resumo: | Heterodera glycines Ichinohe, the soybean cyst nematode (SCN) was detected in Brazil in 1992 and since then it has been causing losses to Brazilian soybean crop yields. SCN populations have great genetic diversity which makes it difficult to manage this disease. The objectives of this research were to investigate the genetic variation of 16 SCN populations sampled in middle western and southeastern Brazil, utilizing random amplified polymorphic DNA (RAPD) techniques, and to establish useful and specific RAPD markers for SCN race 3. RAPD markers demonstrated genetic variability among and within SCN populations, and they could be used for monitoring nematode population dynamics. The OPA-07 primer was a reliable molecular marker for race 3, while electrophoretic profile analysis of DNA fragments amplified with OPA-10 primer detected slight variation within those populations identified as race 3. The SCN population from Chapadão do Céu, GO - sample 2 - was the most genetically distant from the other populations. |
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Genetics and Molecular Biology |
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Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markersHeterodera glycines Ichinohe, the soybean cyst nematode (SCN) was detected in Brazil in 1992 and since then it has been causing losses to Brazilian soybean crop yields. SCN populations have great genetic diversity which makes it difficult to manage this disease. The objectives of this research were to investigate the genetic variation of 16 SCN populations sampled in middle western and southeastern Brazil, utilizing random amplified polymorphic DNA (RAPD) techniques, and to establish useful and specific RAPD markers for SCN race 3. RAPD markers demonstrated genetic variability among and within SCN populations, and they could be used for monitoring nematode population dynamics. The OPA-07 primer was a reliable molecular marker for race 3, while electrophoretic profile analysis of DNA fragments amplified with OPA-10 primer detected slight variation within those populations identified as race 3. The SCN population from Chapadão do Céu, GO - sample 2 - was the most genetically distant from the other populations.Sociedade Brasileira de Genética2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200014Genetics and Molecular Biology v.23 n.2 2000reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572000000200014info:eu-repo/semantics/openAccessSilva,Adailton T. daPenna,Júlio C.V.Goulart,Luiz R.Santos,Maria A. dosArantes,Neylson E.eng2000-09-22T00:00:00Zoai:scielo:S1415-47572000000200014Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2000-09-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
title |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
spellingShingle |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers Silva,Adailton T. da |
title_short |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
title_full |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
title_fullStr |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
title_full_unstemmed |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
title_sort |
Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers |
author |
Silva,Adailton T. da |
author_facet |
Silva,Adailton T. da Penna,Júlio C.V. Goulart,Luiz R. Santos,Maria A. dos Arantes,Neylson E. |
author_role |
author |
author2 |
Penna,Júlio C.V. Goulart,Luiz R. Santos,Maria A. dos Arantes,Neylson E. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva,Adailton T. da Penna,Júlio C.V. Goulart,Luiz R. Santos,Maria A. dos Arantes,Neylson E. |
description |
Heterodera glycines Ichinohe, the soybean cyst nematode (SCN) was detected in Brazil in 1992 and since then it has been causing losses to Brazilian soybean crop yields. SCN populations have great genetic diversity which makes it difficult to manage this disease. The objectives of this research were to investigate the genetic variation of 16 SCN populations sampled in middle western and southeastern Brazil, utilizing random amplified polymorphic DNA (RAPD) techniques, and to establish useful and specific RAPD markers for SCN race 3. RAPD markers demonstrated genetic variability among and within SCN populations, and they could be used for monitoring nematode population dynamics. The OPA-07 primer was a reliable molecular marker for race 3, while electrophoretic profile analysis of DNA fragments amplified with OPA-10 primer detected slight variation within those populations identified as race 3. The SCN population from Chapadão do Céu, GO - sample 2 - was the most genetically distant from the other populations. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572000000200014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.23 n.2 2000 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122377737601024 |