Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes)
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200006 |
Resumo: | Sequential banding analysis (Giemsa-C-banding-Ag NOR) of chromosomes of the common rhea (Rhea americana) was performed. Metaphases were obtained by peripheral blood lymphocyte culture and monolayer embryo cell culture. The diploid chromosome number was 80, different from the 2n = 82 in previous reports. Macrochromosome pairs 1, 2 and 5 were submetacentric and pair 3, subacrocentric. The 4th pair was acrocentric and all of the microchromosomes appeared to be acrocentric, with the exception of a clearly metacentric pair which was fully heterochromatic. The Z was slightly larger than the W, both being acrocentric and C-band negative. Nucleolar organizer regions were observed in the secondary constriction of a microchromosome pair. Correct identification of the NOR-bearing pair was possible only by sequential analyses, Giemsa staining followed by the Ag-NOR technique. |
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Genetics and Molecular Biology |
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Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes)Sequential banding analysis (Giemsa-C-banding-Ag NOR) of chromosomes of the common rhea (Rhea americana) was performed. Metaphases were obtained by peripheral blood lymphocyte culture and monolayer embryo cell culture. The diploid chromosome number was 80, different from the 2n = 82 in previous reports. Macrochromosome pairs 1, 2 and 5 were submetacentric and pair 3, subacrocentric. The 4th pair was acrocentric and all of the microchromosomes appeared to be acrocentric, with the exception of a clearly metacentric pair which was fully heterochromatic. The Z was slightly larger than the W, both being acrocentric and C-band negative. Nucleolar organizer regions were observed in the secondary constriction of a microchromosome pair. Correct identification of the NOR-bearing pair was possible only by sequential analyses, Giemsa staining followed by the Ag-NOR technique.Sociedade Brasileira de Genética1998-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200006Genetics and Molecular Biology v.21 n.2 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000200006info:eu-repo/semantics/openAccessGunski,Ricardo JoséGiannoni,Miriam Luzeng1999-01-06T00:00:00Zoai:scielo:S1415-47571998000200006Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-01-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
title |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
spellingShingle |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) Gunski,Ricardo José |
title_short |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
title_full |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
title_fullStr |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
title_full_unstemmed |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
title_sort |
Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes) |
author |
Gunski,Ricardo José |
author_facet |
Gunski,Ricardo José Giannoni,Miriam Luz |
author_role |
author |
author2 |
Giannoni,Miriam Luz |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Gunski,Ricardo José Giannoni,Miriam Luz |
description |
Sequential banding analysis (Giemsa-C-banding-Ag NOR) of chromosomes of the common rhea (Rhea americana) was performed. Metaphases were obtained by peripheral blood lymphocyte culture and monolayer embryo cell culture. The diploid chromosome number was 80, different from the 2n = 82 in previous reports. Macrochromosome pairs 1, 2 and 5 were submetacentric and pair 3, subacrocentric. The 4th pair was acrocentric and all of the microchromosomes appeared to be acrocentric, with the exception of a clearly metacentric pair which was fully heterochromatic. The Z was slightly larger than the W, both being acrocentric and C-band negative. Nucleolar organizer regions were observed in the secondary constriction of a microchromosome pair. Correct identification of the NOR-bearing pair was possible only by sequential analyses, Giemsa staining followed by the Ag-NOR technique. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47571998000200006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.21 n.2 1998 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122376996257792 |