Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100402 |
Resumo: | Abstract Spontaneous mutations are important players in evolution. Nevertheless, there is a paucity of information about the mutagenic processes operating in most bacterial species. In this work, we implemented two forward mutational markers for studies in Caulobacter crescentus. We confirmed previous results in which A:T → G:C transitions are the most prevalent type of spontaneous base substitutions in this organism, although there is considerable deviation from this trend in one of the loci analyzed. We also investigated the role of dinB and imuC, encoding error-prone DNA polymerases, in spontaneous mutagenesis in this GC-rich organism. Both dinB and imuC mutant strains show comparable mutation rates to the parental strain. Nevertheless, both strains show differences in the base substitution patterns, and the dinB mutant strain shows a striking reduction in the number of spontaneous -1 deletions and an increase in C:G → T:A transitions in both assays. |
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Genetics and Molecular Biology |
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Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentusDinBImuCDnaE2spontaneous mutagenesisCaulobacter crescentusAbstract Spontaneous mutations are important players in evolution. Nevertheless, there is a paucity of information about the mutagenic processes operating in most bacterial species. In this work, we implemented two forward mutational markers for studies in Caulobacter crescentus. We confirmed previous results in which A:T → G:C transitions are the most prevalent type of spontaneous base substitutions in this organism, although there is considerable deviation from this trend in one of the loci analyzed. We also investigated the role of dinB and imuC, encoding error-prone DNA polymerases, in spontaneous mutagenesis in this GC-rich organism. Both dinB and imuC mutant strains show comparable mutation rates to the parental strain. Nevertheless, both strains show differences in the base substitution patterns, and the dinB mutant strain shows a striking reduction in the number of spontaneous -1 deletions and an increase in C:G → T:A transitions in both assays.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100402Genetics and Molecular Biology v.43 n.1 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2018-0283info:eu-repo/semantics/openAccessValencia,Alexy O.Braz,Vânia S.Magalhães,MagnaGalhardo,Rodrigo S.eng2020-03-06T00:00:00Zoai:scielo:S1415-47572020000100402Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-03-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
title |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
spellingShingle |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus Valencia,Alexy O. DinB ImuC DnaE2 spontaneous mutagenesis Caulobacter crescentus |
title_short |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
title_full |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
title_fullStr |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
title_full_unstemmed |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
title_sort |
Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus |
author |
Valencia,Alexy O. |
author_facet |
Valencia,Alexy O. Braz,Vânia S. Magalhães,Magna Galhardo,Rodrigo S. |
author_role |
author |
author2 |
Braz,Vânia S. Magalhães,Magna Galhardo,Rodrigo S. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Valencia,Alexy O. Braz,Vânia S. Magalhães,Magna Galhardo,Rodrigo S. |
dc.subject.por.fl_str_mv |
DinB ImuC DnaE2 spontaneous mutagenesis Caulobacter crescentus |
topic |
DinB ImuC DnaE2 spontaneous mutagenesis Caulobacter crescentus |
description |
Abstract Spontaneous mutations are important players in evolution. Nevertheless, there is a paucity of information about the mutagenic processes operating in most bacterial species. In this work, we implemented two forward mutational markers for studies in Caulobacter crescentus. We confirmed previous results in which A:T → G:C transitions are the most prevalent type of spontaneous base substitutions in this organism, although there is considerable deviation from this trend in one of the loci analyzed. We also investigated the role of dinB and imuC, encoding error-prone DNA polymerases, in spontaneous mutagenesis in this GC-rich organism. Both dinB and imuC mutant strains show comparable mutation rates to the parental strain. Nevertheless, both strains show differences in the base substitution patterns, and the dinB mutant strain shows a striking reduction in the number of spontaneous -1 deletions and an increase in C:G → T:A transitions in both assays. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100402 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2018-0283 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.43 n.1 2020 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122389694513152 |