Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli
Autor(a) principal: | |
---|---|
Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025 |
Resumo: | To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif r) mutant screening and sequencing of the defined region of the rpoB gene in E. coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or 60Co-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG <FONT FACE=Symbol>®</font>TA transitions were significantly increased from 32 to 46, AT <FONT FACE=Symbol>®</font>TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG <FONT FACE=Symbol>®</font>TA transitions, AT <FONT FACE=Symbol>®</font>GC transitions, AT <FONT FACE=Symbol>®</font>TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by 60Co-gamma rays were preferentially GC <FONT FACE=Symbol>®</font>AT and AT <FONT FACE=Symbol>®</font>GC transitions, which totaled 84.31% (43/51). |
id |
SBG-1_2e06a2e64877d393a896e2971300e830 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572004000200025 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia colibase substitutionlow-energy nitrogen ion beam implantation60Co-gamma raysEscherichia coliTo identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif r) mutant screening and sequencing of the defined region of the rpoB gene in E. coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or 60Co-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG <FONT FACE=Symbol>®</font>TA transitions were significantly increased from 32 to 46, AT <FONT FACE=Symbol>®</font>TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG <FONT FACE=Symbol>®</font>TA transitions, AT <FONT FACE=Symbol>®</font>GC transitions, AT <FONT FACE=Symbol>®</font>TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by 60Co-gamma rays were preferentially GC <FONT FACE=Symbol>®</font>AT and AT <FONT FACE=Symbol>®</font>GC transitions, which totaled 84.31% (43/51).Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025Genetics and Molecular Biology v.27 n.2 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000200025info:eu-repo/semantics/openAccessChuan-Xiao,XieAn,XuLi-Jun,WuJian-Min,YaoJian-Bo,YangZeng-Liang,Yueng2004-07-20T00:00:00Zoai:scielo:S1415-47572004000200025Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-07-20T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
title |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
spellingShingle |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli Chuan-Xiao,Xie base substitution low-energy nitrogen ion beam implantation 60Co-gamma rays Escherichia coli |
title_short |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
title_full |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
title_fullStr |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
title_full_unstemmed |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
title_sort |
Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli |
author |
Chuan-Xiao,Xie |
author_facet |
Chuan-Xiao,Xie An,Xu Li-Jun,Wu Jian-Min,Yao Jian-Bo,Yang Zeng-Liang,Yu |
author_role |
author |
author2 |
An,Xu Li-Jun,Wu Jian-Min,Yao Jian-Bo,Yang Zeng-Liang,Yu |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Chuan-Xiao,Xie An,Xu Li-Jun,Wu Jian-Min,Yao Jian-Bo,Yang Zeng-Liang,Yu |
dc.subject.por.fl_str_mv |
base substitution low-energy nitrogen ion beam implantation 60Co-gamma rays Escherichia coli |
topic |
base substitution low-energy nitrogen ion beam implantation 60Co-gamma rays Escherichia coli |
description |
To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif r) mutant screening and sequencing of the defined region of the rpoB gene in E. coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or 60Co-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG <FONT FACE=Symbol>®</font>TA transitions were significantly increased from 32 to 46, AT <FONT FACE=Symbol>®</font>TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG <FONT FACE=Symbol>®</font>TA transitions, AT <FONT FACE=Symbol>®</font>GC transitions, AT <FONT FACE=Symbol>®</font>TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by 60Co-gamma rays were preferentially GC <FONT FACE=Symbol>®</font>AT and AT <FONT FACE=Symbol>®</font>GC transitions, which totaled 84.31% (43/51). |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572004000200025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.27 n.2 2004 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122379036786688 |