Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020 |
Resumo: | Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation. |
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Genetics and Molecular Biology |
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Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia2D electrophoresisElaphe taeniuraMALDI-TOF mass spectrometryproteinSnake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020Genetics and Molecular Biology v.36 n.3 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000300020info:eu-repo/semantics/openAccessChen,LiangXia,HengchuanWang,YitingChen,KepingQin,LvgaoWang,BinYao,QinLi,JunHe,YuanqingZhao,Ermieng2013-08-23T00:00:00Zoai:scielo:S1415-47572013000300020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-08-23T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
spellingShingle |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia Chen,Liang 2D electrophoresis Elaphe taeniura MALDI-TOF mass spectrometry protein |
title_short |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_full |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_fullStr |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_full_unstemmed |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_sort |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
author |
Chen,Liang |
author_facet |
Chen,Liang Xia,Hengchuan Wang,Yiting Chen,Keping Qin,Lvgao Wang,Bin Yao,Qin Li,Jun He,Yuanqing Zhao,Ermi |
author_role |
author |
author2 |
Xia,Hengchuan Wang,Yiting Chen,Keping Qin,Lvgao Wang,Bin Yao,Qin Li,Jun He,Yuanqing Zhao,Ermi |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Chen,Liang Xia,Hengchuan Wang,Yiting Chen,Keping Qin,Lvgao Wang,Bin Yao,Qin Li,Jun He,Yuanqing Zhao,Ermi |
dc.subject.por.fl_str_mv |
2D electrophoresis Elaphe taeniura MALDI-TOF mass spectrometry protein |
topic |
2D electrophoresis Elaphe taeniura MALDI-TOF mass spectrometry protein |
description |
Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572013000300020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.36 n.3 2013 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122385462460416 |