Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia

Detalhes bibliográficos
Autor(a) principal: Chen,Liang
Data de Publicação: 2013
Outros Autores: Xia,Hengchuan, Wang,Yiting, Chen,Keping, Qin,Lvgao, Wang,Bin, Yao,Qin, Li,Jun, He,Yuanqing, Zhao,Ermi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020
Resumo: Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.
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spelling Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia2D electrophoresisElaphe taeniuraMALDI-TOF mass spectrometryproteinSnake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020Genetics and Molecular Biology v.36 n.3 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000300020info:eu-repo/semantics/openAccessChen,LiangXia,HengchuanWang,YitingChen,KepingQin,LvgaoWang,BinYao,QinLi,JunHe,YuanqingZhao,Ermieng2013-08-23T00:00:00Zoai:scielo:S1415-47572013000300020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-08-23T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
title Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
spellingShingle Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
Chen,Liang
2D electrophoresis
Elaphe taeniura
MALDI-TOF mass spectrometry
protein
title_short Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
title_full Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
title_fullStr Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
title_full_unstemmed Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
title_sort Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
author Chen,Liang
author_facet Chen,Liang
Xia,Hengchuan
Wang,Yiting
Chen,Keping
Qin,Lvgao
Wang,Bin
Yao,Qin
Li,Jun
He,Yuanqing
Zhao,Ermi
author_role author
author2 Xia,Hengchuan
Wang,Yiting
Chen,Keping
Qin,Lvgao
Wang,Bin
Yao,Qin
Li,Jun
He,Yuanqing
Zhao,Ermi
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chen,Liang
Xia,Hengchuan
Wang,Yiting
Chen,Keping
Qin,Lvgao
Wang,Bin
Yao,Qin
Li,Jun
He,Yuanqing
Zhao,Ermi
dc.subject.por.fl_str_mv 2D electrophoresis
Elaphe taeniura
MALDI-TOF mass spectrometry
protein
topic 2D electrophoresis
Elaphe taeniura
MALDI-TOF mass spectrometry
protein
description Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572013000300020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.36 n.3 2013
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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