Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley?
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480 |
Resumo: | Abstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the SNP-1,198 allowed the identification of five haplotypes. Short-term soil experiments showed no difference in root growth for most of the accessions containing the 21 bp insertion and T or G-1,198. In contrast, genotypes showing both the 21 bp deletion and G-1,198, with one of them having the 1 kb insertion, showed greater root growth. These results indicate that the SNP was not advantageous or deleterious when genotypes from the same haplotype were compared. The occurrence of the SNP was highly correlated with the 21 bp insertion/deletion that, together with the 1 kb insertion, explained most of the barley tolerance to acidic soil. |
id |
SBG-1_3348fdc391a68a7a308047d8e6580757 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572017000300480 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley?Aluminium tolerancecitrate transporterhaplotypeHordeum vulgaresingle nucleotide polymorphismAbstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the SNP-1,198 allowed the identification of five haplotypes. Short-term soil experiments showed no difference in root growth for most of the accessions containing the 21 bp insertion and T or G-1,198. In contrast, genotypes showing both the 21 bp deletion and G-1,198, with one of them having the 1 kb insertion, showed greater root growth. These results indicate that the SNP was not advantageous or deleterious when genotypes from the same haplotype were compared. The occurrence of the SNP was highly correlated with the 21 bp insertion/deletion that, together with the 1 kb insertion, explained most of the barley tolerance to acidic soil.Sociedade Brasileira de Genética2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480Genetics and Molecular Biology v.40 n.2 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0225info:eu-repo/semantics/openAccessFerreira,Jéssica RossetFaria,Bruna FrancieleComar Junior,MoacyrDelatorre,Carla AndréaMinella,EuclydesPereira,Jorge Fernandoeng2017-06-28T00:00:00Zoai:scielo:S1415-47572017000300480Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-06-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
title |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
spellingShingle |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? Ferreira,Jéssica Rosset Aluminium tolerance citrate transporter haplotype Hordeum vulgare single nucleotide polymorphism |
title_short |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
title_full |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
title_fullStr |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
title_full_unstemmed |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
title_sort |
Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? |
author |
Ferreira,Jéssica Rosset |
author_facet |
Ferreira,Jéssica Rosset Faria,Bruna Franciele Comar Junior,Moacyr Delatorre,Carla Andréa Minella,Euclydes Pereira,Jorge Fernando |
author_role |
author |
author2 |
Faria,Bruna Franciele Comar Junior,Moacyr Delatorre,Carla Andréa Minella,Euclydes Pereira,Jorge Fernando |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Jéssica Rosset Faria,Bruna Franciele Comar Junior,Moacyr Delatorre,Carla Andréa Minella,Euclydes Pereira,Jorge Fernando |
dc.subject.por.fl_str_mv |
Aluminium tolerance citrate transporter haplotype Hordeum vulgare single nucleotide polymorphism |
topic |
Aluminium tolerance citrate transporter haplotype Hordeum vulgare single nucleotide polymorphism |
description |
Abstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the SNP-1,198 allowed the identification of five haplotypes. Short-term soil experiments showed no difference in root growth for most of the accessions containing the 21 bp insertion and T or G-1,198. In contrast, genotypes showing both the 21 bp deletion and G-1,198, with one of them having the 1 kb insertion, showed greater root growth. These results indicate that the SNP was not advantageous or deleterious when genotypes from the same haplotype were compared. The occurrence of the SNP was highly correlated with the 21 bp insertion/deletion that, together with the 1 kb insertion, explained most of the barley tolerance to acidic soil. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2016-0225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.40 n.2 2017 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122387585826816 |